Project description:We created mice, which are deficient for Myc specifically in cardiac myocytes by crossing crossed Myc-floxed mice (Mycfl/fl) and MLC-2VCre/+ mice. Serial analysis of earlier stages of gestation revealed that Myc-deficient mice died prematurely at E13.5-14.5. Morphological analyses of E13.5 Myc-null embryos showed normal ventricular size and structure; however, decreased cardiac myocyte proliferation and increased apoptosis was observed. BrdU incorporation rates were also decreased significantly in Myc-null myocardium. Myc-null mice displayed a 3.67-fold increase in apoptotic cardiomyocytes by TUNEL assay. We examined global gene expression using oligonucleotide microarrays. Numerous genes involved in mitochondrial death pathways were dysregulated including Bnip3L and Birc2. Keywords: wildtype vs Myc-null

2007-06-30 | GSE7162 | GEO

Testis expression in Mus musculus domesticus x Mus musculus musculus hybrid zone mice

Project description:We collected whole genome testis expression data from hybrid zone mice. We integrated GWAS mapping of testis expression traits and low testis weight to gain insight into the genetic basis of hybrid male sterility.

2014-12-04 | GSE61417 | GEO

Effect of Dhps deletion and Citrobacter rodentium on the proteome of colonic epithelial cells

Project description:Dhps<fl/fl> mice and Dhps<fl/fl>;Vil1<cre/+> mice were infected or not with Citrobacter rodentium (10^9 bacteria/mouse) for 14 days, and cell lysates were prepared for analysis. A total of 4 mice per group were used for the following groups: (1) Vil1<+> no C. Rod; (2) Vil1<cre> no C. Rod; (3) Vil1<+> with C. Rod; (4) Vil1<cre> with C. Rod

2025-05-06 | PXD051536 | Pride

Protein disulfide isomerase is essential for osteoblast differentiation in mice

Project description:Protein disulfide isomerase (PDI) is an oxidoreductase responsible for the formation, reduction and isomerization of disulfide bonds of nascent proteins in endoplasmic reticulum (ER). So far, the role of PDI in bone biology has never been characterized using genetically-modified animal models. In this study we generated osteoblast- specific PDI-deficient mice by crossing PDI-floxed (PDIfl/fl) mice with Osx-Cre mice. Compared with their littermate control PDIfl/fl mice, homozygous osteoblast-knockout mice (Osx-Cre/PDIfl/fl) were embryonically lethal, but heterozygous knockout mice (Osx-Cre/PDIfl/wt) displayed significantly pronounced growth retardation and reduced bone length. Besides, the decreases in bone density, osteoblast and osteoclast numbers, collagen fiber content and bone formation rate were observed in Osx-Cre/PDIfl/wt mice. Osteoblast precursors isolated from PDIfl/fl mice were infected with Cre recombinant adenovirus to produce PDI-deficient osteoblasts, followed by induction of differentiation. Osteoblasts deficient of PDI had decreased alkaline phosphatase activity, mineralizing capacity, and differentiation. Quantitative protein mass spectrometry analysis and immunoblotting showed that PDI deficiency markedly decreased the expression of the α-subunits of collagen prolyl 4-hydroxylase (C-P4H), including P4HA1, P4HA2 and P4HA3. These results demonstrate that PDI plays an essential role in osteoblast differentiation and bone formation and is required for the expression of the α-subunit of C-P4H in osteoblasts.

2025-02-19 | PXD060949 | Pride

Bidirectional Introgression between Mus musculus domesticus and Mus spretus.

Project description: Not available

| S-EPMC8784167 | biostudies-literature

Mus musculus

Project description:Expression data from LysMCre/Cre and KLF2∆/∆ (LysMCre/Cre: KLF2 FL/FL) primary peritoneal macrophages treated with LPS for 6hours

| PRJNA136141 | ENA

Mus musculus

Project description:Expression data of LPS-stimulated macrophages in wild-type and LysM-Cre+ Akirin2fl/fl mice

| PRJNA255051 | ENA