Project description:Microdissected ventromedial hypothalamus (VMH) was profiled to identify transcriptional changes after Nkx2-1 ablation in female mice. Total RNA was extracted from conditional mutants (Nkx2-1 f/f; Sf1Cre) and floxed controls (Nkx2-1 f/f) at postnatal day 10 (P10) and profiled using Mouse ref8 v2.0 Illumina chips and reagents (n=4/group). The tissue profiled is microdissected ventromedial hypothalamus from P10 female mice. Controls and mutants are analyzed in quadruplicate.
Project description:Microdissected ventromedial hypothalamus (VMH) was profiled to identify transcriptional changes after Nkx2-1 ablation in female mice. Total RNA was extracted from conditional mutants (Nkx2-1 f/f; Sf1Cre) and floxed controls (Nkx2-1 f/f) at postnatal day 10 (P10) and profiled using Mouse ref8 v2.0 Illumina chips and reagents (n=4/group).
Project description:We aimed to identify genes enriched in Nkx2-1-positive neurons in the dorsomedial hypothalamus (DMH). Using Nkx2-1-CreERT2 mice crossed with Rosa-ZsGreen mice after tamoxifen induction, we collected ZsGreen-marked Nkx2-1-positive neurons from the DMH, ventromedial hypothalamic nucleus (VMH), and arcuate nucleus (Arc) by laser microdissection and compared their gene expression profiles.
Project description:To understand the effects of learning on expression in mouse striatum, we combined an automated operant conditioning chamber (OCC) setup with an efficient RNA-sequencing protocol. We compared 450 striatal expression profiles from 75 mice, e.g., the data contains 6 samples per mouse. Biopsies were taken from both hemispheres, three striatal regions (dorsoventral, dorsomedial, ventromedial striatum) at three learning stages (Early, Intermediate, Late). For each learning stage, there is the same number of samples from paired yoked control mice. There are also samples from control mice that were not kept in OCCs (Naive). The processed data can also be assessed and downloaded from here https://shiny.bio.lmu.de/Dopaloops/
Project description:We used translating ribosome affinity purification (TRAPseq) to profile genetically labeled neurons in the bed nucleus of the stria terminalis (BNST), preoptic area (POA), medial amygdala (MeA) and ventromedial hypothalamus (VMH) from adult male mice, and from adult female mice modeled to be in two distinct stages of the estrus cycle (estrus, sexually receptive, FR; and diestrues, sexually unreceptive, FNR).
Project description:The ventrolateral subdivision of the ventromedial hypothalamus (VMHvl) is essential for innate social behaviors including aggression and mating in female mice at different reproductive states. However, the female VMHvl transcriptomes in different reproducive states and their relationship behavioral functions are unknown. We performed single-cell RNA 10x sequencing and investigated correspondence between transcriptomic identity in different reproductive states and behavioral activation. Immediate early gene analysis from our Act-seq dataset identified that distinct T-types function in female aggression and mating behaviors.
Project description:We identified genes expressed in mouse liver that are regulated by Cux2, a highly female-specific liver transcription factor whose expression is regulated by sex-dependent plasma GH patterns. Using siRNA to knockdown Cux2 expression in female liver, we show that female specific genes are predominantly repressed by Cux2 knockdown. In contrast, similar numbers of male-biased genes are repressed as are induced by Cux2 knockdown. A scrambled, non-specific siRNA was used as a control. (Published in Molec Cell Biology, TL Conforto et al, 2012) Liver RNA isolated from the following 3 groups of mice was used in the present study: (1) 8 wk old female mice treated with non-specific siRNA control (n = 13; 6 or 7 per each pool); (2) 8 wk old female mice treated with Cux2 siRNA and euthanized 5 days later (n = 5; 2 or 3 per each pool); (3) 8 wk old female mice treated with Cux2 siRNA and euthanized 8 days later (n = 4; 2 per each pool). These RNA pools were used in two separate sets of competitive hybridization experiments: 1) 8 wk non-specific siRNA treated vs. 8 wk Cux2 siRNA treated for 5 days; 2) 8 wk non-specific siRNA treated vs. 8 wk Cux2 siRNA treated for 8 days. Fluorescent labeling of RNA and hybridization of the Alexa 555-labeled (green) and Alexa 647-labeled (red) RNA samples to Agilent Mouse Gene Expression 4x44k v1 microarrays (Agilent Technology, Palo Alto, CA; catalog # G4122F-014868) were carried out, with dye swapping for each of the two hybridization experiments to eliminate dye bias. Two microarrays, one for each mixed cDNA sample, were hybridized for each of the two fluorescent reverse pairs, giving a total of 4 microarrays.
