Project description:The purpose of this study is to determine whether the presence of pathogenic Escherichia coli in colon is associated with psychiatric disorders.
Project description:Neonatal meningitis caused by Escherichia coli (NMEC) is a leading cause of morbidity and mortality in newborns, and its pathogenesis relies on the ability of the bacterium to adapt and survive in diverse host environments. Despite advances in neonatal care, significant gaps remain in our understanding of how NMEC reprogram their transcriptome to survive in physiologically relevant niches. This study investigated the transcriptomic profiles of E. coli strain RS218 (O18:H7:K1) in four under host-relevant environment —colonic fluid (CF), serum (S), human brain endothelial cells (HBECs) and cerebrospinal fluid (CSF)—to mimic the infection landscape of neonatal meningitis. High-throughput RNA sequencing (RNA-seq) was performed to profile NMEC’s transcriptomic responses in each niche, and differential gene expression analyses were conducted to identify enriched pathways.
Project description:This project used biotin pull-down to screen membrane proteins with FH binding function from the membrane proteins of Escherichia coli RS218. It contained an experimental group (SHIYANZU1) and a control group (DUIZHAOZU)..
Project description:Despite the characterization of many aetiologic genetic changes. The specific causative factors in the development of sporadic colorectal cancer remain unclear. This study was performed to detect the possible role of Enteropathogenic Escherichia coli (EPEC) in developing colorectal carcinoma.
Project description:Human cerebral microvascular endothelial cells (HCMECs) were infected with extraintestinal pathogenic Escherichia coli (ExPEC) strain RS218 at a multiplicity of infection (MOI) of 1 for 3 hours. Proteomic analysis was performed to evaluate host cellular responses. Infected samples were compared to uninfected controls (MOCK) to identify differentially expressed proteins associated with ExPEC infection.
Project description:we designed a CRISPR-based chromosome-doubling technique to construct an artificial diploid Escherichia coli cell. The stable diploid E. coli was confirmed by quantitative PCR and third-generation genome sequencing.
