Project description:We performed RNA sequencing (RNA-seq) and m6A methylated RNA immunoprecipitation sequencing (MeRIP-seq) in control and Zfp217 knockout 3T3L1 cells with MDI treatment for 0d and 2d Loss-of-function study demonstrates that Zfp217 deficiency impaired adipogenesis together with a global increase of m6A modification in 3T3L1cells. To gain an overview of the global role of Zfp217 in adipogenesis, we performed RNA sequencing (RNA-seq) in control and Zfp217 knockout 3T3L1 cells with MDI treatment for 0d and 2d and identified 12,188 and 11,566 different expressed genes (DEG) for 0d and 2d, respectively. Next, to elucidate the mechanism by which Zfp217 regulates m6A restriction, m6A methylated RNA immunoprecipitation sequencing (MeRIP-seq) was used to analyze the m6A mRNA methylation in control and Zfp217 knockout 3T3L1 cells with MDI treatment for 0d and 2d, and identified 3149 and 294 of m6A peaks experienced an increase in m6A RNA modification after Zfp217 depletion, respectively.
Project description:Many transcriptional and epigenetic networks must be integrated to maintain self-renewal and pluripotency in embryonic stem cells (ESCs) and to enable induced pluripotent stem cell (iPSC) reprogramming. Here, we explore the role of Zfp217 as a key transcriptional factor in maintaining ES cell self-renewal by performing meRIP analysis in control and Zfp217-depleted mouse stem cells. Examination of m6A levels from total RNA in control and Zfp217 shRNA infected mouse stem cells
Project description:We use comprehensive and unsupervised transcriptome analyses to provide molecular classifications of sensory neurons in the mouse geniculate ganglion. 96 neurons were isolated on a C1 Fluodigm chip, underwent RNA-Seq, and iteratively clustered into sub-classes.
Project description:Comprehensive RNA-seq experiments in control and zfp217 shRNA infected cells delineate a Zfp217-driven network required for ES cell self-renewal.
Project description:Many transcriptional and epigenetic networks must be integrated to maintain self-renewal and pluripotency in embryonic stem cells (ESCs) and to enable induced pluripotent stem cell (iPSC) reprogramming. Here, we explore the role of Zfp217 as a key transcriptional factor in maintaining ES cell self-renewal by permorming genome-wide ChIP-Seq analyses. Examination of Zfp217 binding profiling by high throughput sequencing in mouse stem cells