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Mus musculus

ABSTRACT: Comprehensive role of Zfp217 in m6A methylation [MeRIP-seq]

PROVIDER: PRJNA274809 | ENA |

REPOSITORIES: ENA

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			Action	DRS
	SRR1793130_1.fastq.gz	Fastqsanger.gz
	SRR1793130_2.fastq.gz	Fastqsanger.gz
	SRR1793131_1.fastq.gz	Fastqsanger.gz
	SRR1793131_2.fastq.gz	Fastqsanger.gz
	SRR1793132_1.fastq.gz	Fastqsanger.gz

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Zfp217 orchestrates m6A mRNA methylation and gene expression to promote adipogenic differentiation

Project description:We performed RNA sequencing (RNA-seq) and m6A methylated RNA immunoprecipitation sequencing (MeRIP-seq) in control and Zfp217 knockout 3T3L1 cells with MDI treatment for 0d and 2d Loss-of-function study demonstrates that Zfp217 deficiency impaired adipogenesis together with a global increase of m6A modification in 3T3L1cells. To gain an overview of the global role of Zfp217 in adipogenesis, we performed RNA sequencing (RNA-seq) in control and Zfp217 knockout 3T3L1 cells with MDI treatment for 0d and 2d and identified 12,188 and 11,566 different expressed genes (DEG) for 0d and 2d, respectively. Next, to elucidate the mechanism by which Zfp217 regulates m6A restriction, m6A methylated RNA immunoprecipitation sequencing (MeRIP-seq) was used to analyze the m6A mRNA methylation in control and Zfp217 knockout 3T3L1 cells with MDI treatment for 0d and 2d, and identified 3149 and 294 of m6A peaks experienced an increase in m6A RNA modification after Zfp217 depletion, respectively.

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Comprehensive role of Zfp217 in m6A methylation [MeRIP-seq]

Project description:Many transcriptional and epigenetic networks must be integrated to maintain self-renewal and pluripotency in embryonic stem cells (ESCs) and to enable induced pluripotent stem cell (iPSC) reprogramming. Here, we explore the role of Zfp217 as a key transcriptional factor in maintaining ES cell self-renewal by performing meRIP analysis in control and Zfp217-depleted mouse stem cells. Examination of m6A levels from total RNA in control and Zfp217 shRNA infected mouse stem cells

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Project description:Comprehensive role of Zfp217 in m6A methylation

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Adenosine methylation is widespread throughout the transcriptome and prominent within 3' UTRs

Project description:Here, we use a novel technique for locating regions of N6-adenosine methylation (m6A) throughout the transcriptome and present a profile of m6A sites in the mouse brain. Our use of methylated RNA immunoprecipitation combined with RNA-seq (MeRIP-Seq) identifies thousands of RNAs which contain m6A sites. In addition, we find that regions of m6A formation are particularly enriched near stop codons, which might provide clues into the potential funciton of this highly prevalent RNA modificaiton. Examination of m6A sites in murine brain RNA.

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