Project description:Analysis of the effect of vitamin C on gene expression regulation in iTreg cells. Results provide important information of the response of iTreg cells to vitamin C.
Project description:Analysis of the effect of vitamin C on gene expression regulation in iTreg cells. Results provide important information of the response of iTreg cells to vitamin C. Total RNA obtained from iTreg cells cultured for 5 days with or without vitamin C compared to natural Treg and naïve CD4+ T cells
Project description:PURPOSE: To provide a detailed gene expression profile of the normal postnatal mouse cornea. METHODS: Serial analysis of gene expression (SAGE) was performed on postnatal day (PN)9 and adult mouse (6 week) total corneas. The expression of selected genes was analyzed by in situ hybridization. RESULTS: A total of 64,272 PN9 and 62,206 adult tags were sequenced. Mouse corneal transcriptomes are composed of at least 19,544 and 18,509 unique mRNAs, respectively. One third of the unique tags were expressed at both stages, whereas a third was identified exclusively in PN9 or adult corneas. Three hundred thirty-four PN9 and 339 adult tags were enriched more than fivefold over other published nonocular libraries. Abundant transcripts were associated with metabolic functions, redox activities, and barrier integrity. Three members of the Ly-6/uPAR family whose functions are unknown in the cornea constitute more than 1% of the total mRNA. Aquaporin 5, epithelial membrane protein and glutathione-S-transferase (GST) omega-1, and GST alpha-4 mRNAs were preferentially expressed in distinct corneal epithelial layers, providing new markers for stratification. More than 200 tags were differentially expressed, of which 25 mediate transcription. CONCLUSIONS: In addition to providing a detailed profile of expressed genes in the PN9 and mature mouse cornea, the present SAGE data demonstrate dynamic changes in gene expression after eye opening and provide new probes for exploring corneal epithelial cell stratification, development, and function and for exploring the intricate relationship between programmed and environmentally induced gene expression in the cornea. Keywords: other
Project description:Platelets are a rich source of many cytokines and chemokines including transforming growth factor β-1 (TGFβ1). TGFβ1 is required to convert conventional CD4+ T (Tconv) cells into induced regulatory T (iTreg) cells that express the transcription factor Foxp3. To explore whether other platelet contents will affect the properties of TGFβ induced Treg cell, we used platelet lysate that contain many other cytokines and chemokines besides TGFβ1 (pltTGFβ) to induce Foxp3 expression (pltTGFb-iTreg) from conventional CD4+ T (Tconv) cells. We used purified TGFβ1 to induce Treg (purTGFβ-iTreg) cells as a control. Gene expression profiles in iTreg cells were analyzed by microarray asay.
