Browse
Submit Data
Databases
API
Help

Dataset Information

0 Views

0 Connections

0 Citations

0 Reanalyses

0 Downloads

Omics score: 0

Mycobacterium tuberculosis variant bovis BCG

ABSTRACT: Impact of in vitro evolution on antigenic diversity of Mycobacterium bovis bacillus Calmette-Guerin (BCG).

PROVIDER: PRJNA280619 | ENA |

REPOSITORIES: ENA

ACCESS DATA

Json Xml

Dataset's files

Source:

			Action	DRS
	SRR1957685.fastq.gz	Fastqsanger.gz

Items per page:

1 - 1 of 1

Similar Datasets

Transcriptional signature of BCG vaccination

Project description:The century-old Mycobacterium bovis Bacillus Calmette-Guerin (BCG) remains the only licensed vaccine against tuberculosis (TB). Despite this, there is still a lot to learn about the immune response induced by BCG, both in terms of phenotype and specificity. Here, we investigated the BCG-specific gene expression changes induced in PBMCs and CD4 memory T cells by BCG in individuals pre- and 8m post vaccination. We also determined whether reactivity against a peptide pool defined in individuals with controlled latent TB infection (MTB300), and with peptides homologous to peptides found in BCG, was boosted following BCG vaccination.

2021-12-06 | GSE156422 | GEO

Cultivation impact on the protein expression profile of Mycobacterium bovis BCG strains Moreau and Pasteur

Project description:The BCG vaccine (Bacille Calmette-Guerin), only prophylactic measure against tuberculosis (TB), was obtained in the early twentieth century by Calmette and Guérin after 231 passages of a M. bovis clinical isolate in medium containing glycerin and bovine bile. Its protective efficacy against pulmonary TB in adults varies from 0-80% and the genetic differences among vaccines strains used worldwide contribute to this variation. The Brazilian vaccine strain, BCG Moreau, is considered a primitive strain and more immunogenic, closer to the original BCG when compared to newer strains, such as BCG Pasteur (reference strain). The characterization of BCG sub-strains can contribute not only to a better understanding of the vaccine and its protective effect, but also, in elucidating how different BCG culture conditions may contribute to the impact on the host's immune response. Thus, we aimed to characterize the differences in gene expression through the intracellular proteomic profile of BCG Moreau and Pasteur strains, cultivated in Sauton or 7H9 medium, using the methodology of two-dimensional electrophoresis (2DE) and mass spectrometry.

2025-10-23 | PXD030179 | Pride

Mycobacterium tuberculosis variant bovis BCG strain:Bacillus Calmette-Guerin | isolate:M1_S49

Project description:Mycobacterium tuberculosis variant bovis BCG strain:Bacillus Calmette-Guerin | isolate:M1_S49 Genome sequencing and assembly

| PRJNA498011 | ENA

Microarray Analysis of Toxicogenomic Effects of Triclosan on Mycobacterium bovis BCG

Project description:Global gene expression analysis of Mycobacterium bovis BCG following Triclosan treatment using Affymetrix GeneChip arrays. Results from this study provide insight into the molecular mechanisms underlying the cellular response of Mycobacterium bovis BCG to Triclosan

2012-12-25 | GSE33800 | GEO

Exploring the potential role of moonlighting function of the surface-associated proteins from Mycobacterium bovis BCG Moreau and Pasteur by comparative proteomic

Project description:Tuberculosis (TB) is one of major causes of death worldwide. Bacillus Calmette-Guerin (BCG) is the only licensed TB vaccine and its inability to protect against adult pulmonary TB can be due to genetic differences among strains described since the 1940s. In this work, we compared the proteomic profile of the surface-associated proteins from M. bovis BCG Moreau, the Brazilian vaccine strain, and the BCG Pasteur reference strain. The methodology used was 2D-gel electrophoresis combined with mass spectrometry techniques (MALDI-TOF/TOF). We identified 115 proteins. Of these, 24 proteins showed differential expression between the two BCG strains. Furthermore, 27 proteins previously described as displaying moonlighting function were identified, 8 of these proteins showed variation in abundance comparing BCG Moreau to Pasteur and 2 of them presented two different domain hits.

2019-05-15 | PXD006141 | Pride

Mycobacterium tuberculosis variant bovis BCG

Project description:Mycobacterium bovis BCG Genome sequencing

| PRJNA266372 | ENA

BCG vaccine strains lack narK2 and narX induction and exhibit altered phenotypes during dormancy

Project description:Mycobacterium tuberculosis is the causative agent of tuberculosis, a disease that affects one-third of the world’s population. The sole extant vaccine for tuberculosis is the live attenuated Mycobacterium bovis bacille Calmette-Guerin (BCG). We examined 13 representative BCG strains from around the world to ascertain their ability to express DosR-regulated dormancy antigens. These are known to be recognized by T-cells of M. tuberculosis infected individuals, especially those harboring latent infections. Differences in expression of these antigens could be valuable for use as diagnostic markers to distinguish BCG vaccination from latent tuberculosis. We determined that all BCG strains were defective for induction of two dormancy genes, narK2 (Rv1737c) and narX (Rv1736c). NarK2 is known to be necessary for nitrate respiration during anaerobic dormancy. Analysis of the narK2/X promoter region revealed a base substitution mutation in all tested BCG strains and M. bovis in comparison to the M. tuberculosis sequence. We also show that nitrate reduction by BCG strains during dormancy was greatly reduced compared to M. tuberculosis and varied between tested strains. Several dormancy regulon transcriptional differences were also identified among the strains, as well as variation in their growth and survival. These findings demonstrate defects in DosR regulon expression during dormancy and phenotypic variation between commonly used BCG vaccine strains. 12 different BCG strains were examined as well as M. tuberculosis H37Rv and M. bovis. Two arrays per strain were analyzed, one with the addition of nitric oxide and the other utilizing hypoxia treatment, both conditions shown to induce expression of the dormancy regulon. The reference sample for each array was log phase M. tuberculosis H37Rv.

2008-05-03 | E-GEOD-11315 | biostudies-arrayexpress

Microarray Analysis of Toxicogenomic Effects of Hydrogen Peroxide on Mycobacterium bovis BCG

Project description:In the present study, we employed Affymetrix Mycobacterium bovis BCG GeneChip arrays to investigate the dynamics of global gene expression profiles during the cellular response of Mycobacterium bovis BCG to hydrogen peroxide, which involved initial growth inhibition and metabolism. Keywords: Transcriptome study

2009-01-20 | GSE14272 | GEO

Microarray Analysis of Toxicogenomic Effects of Sodium Hypochlorite on Mycobacterium bovis BCG

Project description:In the present study, we employed Affymetrix Mycobacterium bovis BCG GeneChip arrays to investigate the dynamics of global gene expression profiles during the cellular response of Mycobacterium bovis BCG to Sodium Hypochlorite, which involved initial growth inhibition and metabolism. Keywords: Transcriptome study

2008-12-01 | GSE13423 | GEO

Microarray Analysis of Toxicogenomic Effects of peracetic acid on Mycobacterium bovis BCG

Project description:In the present study, we employed Affymetrix Mycobacterium bovis BCG GeneChip arrays to investigate the dynamics of global gene expression profiles during the cellular response of Mycobacterium bovis BCG to Peracetic acid, which involved initial growth inhibition and metabolism. Keywords: Transcriptome study

2009-03-31 | GSE15023 | GEO

OmicsDI is part of the ELIXIR infrastructure

OmicsDI is an Elixir interoperability service. Learn more ›

Tweets

OmicsDI Databases

PRIDE
PeptideAtlas
MassIVE
JPOST Repository
Physiome Model Repository

EGA
EVA
ENA
LINCS
PAXDB
Cell Collective

MetaboLights
Metabolomics Workbench
MetabolomeExpress
GNPS
BioModels
FAIRDOMHub

ArrayExpress
dbGaP
ExpressionAtlas
GEO
NODE

Information

Databases
Help
API
Contact us
Code on GitHub
Terms of use
Submit Data