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Caenorhabditis elegans

ABSTRACT: Genome-wide RNA Pol II-CTD occupancy in wild type and mutant worms

PROVIDER: PRJNA282022 | ENA |

REPOSITORIES: ENA

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			Action	DRS
	SRR1993090.fastq.gz	Fastqsanger.gz
	SRR1993091.fastq.gz	Fastqsanger.gz
	SRR1993092.fastq.gz	Fastqsanger.gz
	SRR1993093.fastq.gz	Fastqsanger.gz
	SRR1993094.fastq.gz	Fastqsanger.gz

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Genome-wide RNA Pol II-CTD occupancy in wild type and mutant worms

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Genome-wide RNA Pol II-CTD occupancy in wild type and mutant worms

Project description:We used RNA Pol II-CTD occupancy assay to identify mRNA isoform switch in mutant animals compared to wild type animals. Examination of RNA Pol II-CTD Ser5P recognized by 4H8 in wild type, sydn-1(0), ssup-72(0) and ssup-72(0);sydn-1(0)

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Project description:The carboxyl-terminal domain (CTD) of the largest subunit of RNA polymerase II (Pol II) orchestrates dynamic recruitment of specific cellular machines during different stages of transcription. Signature phosphorylation patterns of Y1S2P3T4S5P6S7 heptapeptide repeats of the CTD engage specific “readers.” While phospho-Ser5 and phospho-Ser2 marks are ubiquitous, phospho-Thr4 is reported to only impact specific genes. Here, we investigate the genome-wide occupancy of Pol II, phospho-Thr4, and key reader Rtt103 in WT and CTD-mutant strains of S. cerevisiae.

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