Project description:This project utilized CRISPR-assisted RNA-protein interaction detection (CARPID) combined with mass spectrometry to identify proteins interacting with the long non-coding RNA (lncRNA) TUG1 in HEK293T cells. TUG1 preferentially interacts with known R-loop-associated proteins, particularly under Camptothecin (CPT) treatment. Additionally, we identified interacting proteins for the lncRNAs MALAT1 to validate the binding specificity of TUG1.
Project description:Hypertrophic cardiomyopathy (HCM) represents one of the most common heritable heart diseases. However, the signalling pathways and regulatory networks underlying the pathogenesis of HCM remain largely unknown. Here, we present a strand-specific RNA-seq dataset for both coding and lncRNA profiling in myocardial tissues from 28 HCM patients and 9 healthy donors. This dataset constitutes a valuable resource for the community to examine the dysregulated coding and lncRNA genes in HCM versus normal conditions.
Project description:proteins interacting with the long non-coding RNA (lncRNA) LINC01940. We performed RNA Immunoprecipitation (RIP) using an antibody specific to LINC01940 in gastric cancer cells. The immunoprecipitated RNA-protein complexes were then subjected to immunoprecipitation followed by mass spectrometry (IP/MS) to identify the associated proteins.
Project description:The expression of the long non-coding RNAs (lncRNAs) in the active tuberculosis patients,multidrug-resistant tuberculosis patients,cured tuberculosis subjects and healthy controls were scanned using lncRNA microarray.The Arraystar Human LncRNA Microarray version 4.0 (Agilent Technologies) was used,containing 40173 lncRNA probes and 20730 mRNA probes for the detection of lncRNAs and mRNAs in the sample. Non-coding RNAs and mRNAs profiling by array
Project description:Collecting duct carcinoma (CDC) is a rare and aggressive renal malignancy with limited therapeutic options. In this study, long non-coding RNA (lncRNA) expression profiles were analyzed in two CDC cases with differential responses to immune checkpoint inhibitor (ICI) therapy. Case 1 showed a clinical response to ICI treatment, whereas Case 2 was refractory. Tumor and matched normal FFPE kidney tissues were subjected to lncRNA expression profiling using the Arraystar Human LncRNA Microarray v5.0 platform to explore potential molecular differences associated with ICI response.
Project description:This dataset contains label-free LC–MS/MS proteomics results from endogenous RNA pulldown assays performed in WI-38 cells to identify RNA-binding proteins associated with targeted long non-coding RNAs (odd/even probe sets) compared with a scramble control. The resulting protein identification/quantification matrix is provided to support downstream analysis of lncRNA–protein interaction partners.
2026-02-10 | PXD074193 | Pride
Project description:Long QTc in hypertrophic cardiomyopathy
Project description:We report the application of RNA-sequencing technology for high-throughput profiling of Drosophila that express clinical variants of feline-MyBPC3 associated with Hypertrophic Cardiomyopathy (HCM).
