Project description:Effect of TNF-alpha on microRNAs levels in Human Umbilical Endothelial Cells (HUVECs). HUVEC that were treated or not for 2 or 24 hours with TNF (10 ng/ml). Duplicate samples (1 or 2) of two different isolations of HUVEC (A or B)
Project description:A panel of 17 human melanoma cell lines with known BRAF and NRAS mutation status was stimulated with TNF-alpha for 72 hours. The goal of the study was to correlate the transcriptional response in BRAF versus NRAS mutated melanoma cell lines. Total RNA was obtained from a panel of 17 human melanoma cell lines treated for 72 hours with TNF-alpha or left untreated. Gene expression profiling was done using the Illumina Human HT12 v4 platform.
Project description:A panel of 17 human melanoma cell lines with known BRAF and NRAS mutation status was stimulated with TNF-alpha for 72 hours. The goal of the study was to correlate the transcriptional response in BRAF versus NRAS mutated melanoma cell lines.
Project description:To investigate effects of GLI1, GLI3, and GLI1/3 knockdown on rhematoid arthritis synovial fibroblasts (RASFs) stimulated with TNF-alpha, we pre-treated RASFs (n = 3) with siControl (siCtrl) or siGLI1, siGLI3, or GLI1/3 for 72 hours, and then treated them with TNF-alpha (10 ng/mL) for 24 hours and conducted RNA-seq analysis.
