Ontology highlight
ABSTRACT:
PROVIDER: PRJNA295604 | ENA |
REPOSITORIES: ENA
Dataset's files
| Action | DRS | |||
|---|---|---|---|---|
| SRR2416670.fastq.gz | Fastqsanger.gz | |||
| SRR2416212_1.fastq.gz | Fastqsanger.gz | |||
| SRR2416212_2.fastq.gz | Fastqsanger.gz | |||
| SRR2416682_1.fastq.gz | Fastqsanger.gz | |||
| SRR2416682_2.fastq.gz | Fastqsanger.gz |
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Similar Datasets
Project description:Toxoplasma gondii (T. gondii) is an opportunistic parasite that can infect the central nervous system, causing severe toxoplasmosis and behavioral cognitive impairment. Mortality is high in immunocompromised individuals with toxoplasmosis, most commonly due to reactivation of infection in the central nervous system (CNS). There are still no effective vaccines and drugs for the prevention and treatment of toxoplasmosis. There are five developmental stages for T. gondii to complete life cycle, of which the tachyzoite and bradyzoite stages are the key to the acute and chronic infection. In this study, to better understanding of how T. gondii interacts with the host central nervous system at different stages of infection, we constructed acute and chronic infection models of T. gondii in astrocytes, and used lab-free proteomics to detect the proteome changes before and after infection, respectively.
2023-08-25 | PXD038919 | Pride
Project description:Toxoplasma gondii is a ubiquitous apicomplexan protozoan parasite that can infect all warm-blooded animals and lead to toxoplasmosis. Discovering efficient methods for diagnosing T. gondii infection and developing vaccine candidates are essential for controlling this disease. Circulating antigens (CAg) are reliable diagnostic indicators of acute infection and highly immunogenic compounds that induce protective immunity. We aimed to identify the CAg spectrum in infected mouse serum. Among 31 identified CAg, we evaluated the ability of RuvB-like helicase (TgRuvBL1), ribonuclease (TgRNaseH1), and ribosomal protein RPS2 (TgRPS2) to protect against T. gondii infection. These three proteins were found in the serum of infected mice. Vaccination with rTgRuvBL1 or rTgRPS2 elicited powerful humoral and cellular immune responses in mice, as levels of total IgG, IgG2a, IgG1, and CD4+ and CD8+ T lymphocytes were significantly increased. The production of proinflammatory gamma-interferon, interleukin-2, and interleukin-12 was also significantly increased. The survival of mice immunized with rTgRuvBL1 (10.0 ± 0.30 days) or rTgRPS2 (11.5 ± 0.34 days) was significantly prolonged after challenge with virulent T. gondii RH strain (P < 0.05 vs. control). The three proteins are potential diagnostic candidates for acute T. gondii and TgRuvBL1 and TgRPS2 might serve as candidate vaccine antigens against toxoplasmosis.
2021-01-04 | PXD021938 | Pride
Project description:Tymoshenko2015 - Genome scale metabolic model
- ToxoNet1
This model is described in the article:
Metabolic Needs and
Capabilities of Toxoplasma gondii through Combined
Computational and Experimental Analysis.
Tymoshenko S, Oppenheim RD, Agren R,
Nielsen J, Soldati-Favre D, Hatzimanikatis V.
PLoS Comput. Biol. 2015 May; 11(5):
e1004261
Abstract:
Toxoplasma gondii is a human pathogen prevalent worldwide
that poses a challenging and unmet need for novel treatment of
toxoplasmosis. Using a semi-automated reconstruction algorithm,
we reconstructed a genome-scale metabolic model, ToxoNet1. The
reconstruction process and flux-balance analysis of the model
offer a systematic overview of the metabolic capabilities of
this parasite. Using ToxoNet1 we have identified significant
gaps in the current knowledge of Toxoplasma metabolic pathways
and have clarified its minimal nutritional requirements for
replication. By probing the model via metabolic tasks, we have
further defined sets of alternative precursors necessary for
parasite growth. Within a human host cell environment, ToxoNet1
predicts a minimal set of 53 enzyme-coding genes and 76
reactions to be essential for parasite replication.
Double-gene-essentiality analysis identified 20 pairs of genes
for which simultaneous deletion is deleterious. To validate
several predictions of ToxoNet1 we have performed experimental
analyses of cytosolic acetyl-CoA biosynthesis. ATP-citrate
lyase and acetyl-CoA synthase were localised and their
corresponding genes disrupted, establishing that each of these
enzymes is dispensable for the growth of T. gondii, however
together they make a synthetic lethal pair.
This model is hosted on
BioModels Database
and identified by:
MODEL1504280000.
To cite BioModels Database, please use:
BioModels Database:
An enhanced, curated and annotated resource for published
quantitative kinetic models.
To the extent possible under law, all copyright and related or
neighbouring rights to this encoded model have been dedicated to
the public domain worldwide. Please refer to
CC0
Public Domain Dedication for more information.
2015-07-07 | MODEL1504280000 | BioModels
Project description:Toxoplasmosis is a major health issue worldwide especially for immune-deficient individuals and the offspring of newly infected mothers. It is caused by a unicellular intracellular parasite called Toxoplasma gondii. Although the drugs commonly used to treat toxoplasmosis are efficient, they present serious side effects and adverse events are common. Therefore, there is a need for the discovery of new compounds with potent anti-T. gondii activity. We have tested compounds designed to target enzymes that are involved in the epigenetic regulation of gene expression. Among the most active compounds, we identified a HDAC inhibitor that shows an IC50 of around 30 nM with a selectivity index of more than 100. MC1742 is active at inhibiting the growth of the parasite in vitro but also at preventing the consequences of the acute disease in vivo. This compound induces a hyper-acetylation of histones while acetylated tubulin level remains unchanged. After MC1742 treatment, the parasite expression profile is profoundly changed with the activation of genes preferentially expressed in the sexual stages that are normally repressed at the tachyzoite stage. These findings suggest that this compound disturbs the T. gondii gene expression program, inducing parasite death.
2024-05-22 | GSE175919 | GEO
Project description:Toxoplasma gondii is a ubiquitous pathogen of warm-blooded animals and belongs to the phylum of apicomplexan parasites. Apicomplexans cause diseases of high mortality, including toxoplasmosis, malaria, and cryptosporidiosis. Cyclic nucleotide-dependent protein kinases in this divergent family of parasites play crucial roles in pathogenesis and spread. Here, we conduct a phosphoproteomics experiment after T. gondii parasites are depleted of the protein kinase A regulatory subunit (PKA R), resulting in up-regulation of the PKA catalytic subunit 1 (PKA C1).
2024-07-12 | PXD044398 | Pride
Project description:Two samples, 0hr and 72hr, were used to generate tachyzoite and bradyzoite transcriptional data from tissue-cultured Toxoplasma gondii strain Prugniaud, respectively.
2010-06-15 | GSE22258 | GEO
Project description:Lysine lactylation (Kla) is a new posttranslational modification (PTM) identified in histone and nonhistone proteins of several eukaryotic cells that directly activate gene expression and DNA replication. However, very little is known about their scope and cellular distribution in apicomplexan parasites that are important to public and animal health. Toxoplasma gondii, the agent of toxoplasmosis, is one of obligate intracellular apicomplexan parasite that can infect all kinds of nucleated cells of animals and humans. Using this parasite as model organism, herein, we produced the first global lysine lactylome profile through LC-MS/MS. Overall, a total of 983 Kla sites occurred on 523 lactylated proteins were identified in Toxoplasma tachyzoites, the acute toxoplasmosis-causing stage. Bioinformatics analysis revealed that these lactylated proteins are evolutionarily conserved and involved in a wide variety of cellular functions such as energy metabolism, gene regulation and protein biosynthesis. Moreover, the results from subcellular localization analysis and IFA showed that the majority of lactylated T. gondii proteins localized in the nucleus, indicating a potential impact of Kla on gene regulation. Notably, an extensive batch of parasite-specific proteins unique to Apicomplexa is lactylated in T. gondii. Our findings revealed that Kla was widespread in the early branching eukaryotic cell, and that lactylated proteins, including a crowd of unique parasite proteins, were involved in a remarkably diverse array of cellular functions. These valuable data will improve our understanding of the evolution of Kla while potentially providing novel therapeutic avenues.
2022-06-09 | PXD031526 | Pride
Project description:Analysis of Ly6Chi monocytes from small intestine lamina propria (SILP) and blood of day 8 Toxoplasma gondii infected mice at gene expression level. The hypothesis tested in the present study was that Ly6Chi monocytes from SILP have altered expression of regulatory factors to blood monocytes. Results provide important information on the regulatory to effector balance of genes expressed by Ly6Chi monocytes during an acute inflammatory response. Ly6Chi inflammatory monocytes were sorted by FACS from the blood or small intestine lamina propria (SILP) of Toxoplasma gondii infected C57BL/6 mice. Cells were isolated at day 8 after infection and total RNA obtained from sorted populations. Three biological replicates were acquired for both blood and SILP from pooled animals.
2013-05-25 | E-GEOD-46685 | biostudies-arrayexpress
Project description:Toxoplasma gondii, the causative agent of toxoplasmosis, is an obligate intracellular protozoan parasite that resides inside a parasitophorous vacuole. During infection, Toxoplasma actively remodels the transcriptome of its hosting cells with profound and coupled impact on the host immune response. We report that Toxoplasma secretes GRA24, a novel dense granule protein which traffics from the vacuole to the host cell nucleus. Once released into the host cell, GRA24 has the unique ability to trigger prolonged autophosphorylation and nuclear translocation of the host cell p38M-NM-1 MAP kinase. This noncanonical kinetics of p38M-NM-1 activation correlates with the up-regulation of the transcription factors Egr-1 and c-Fos and the correlated synthesis of key proinflammatory cytokines, including interleukin-12 and the chemokine MCP-1, both known to control early parasite replication in vivo. Remarkably, the GRA24-p38M-NM-1 complex is defined by peculiar structural features and uncovers a new regulatory signaling path distinct from the MAPK signaling cascade and otherwise commonly activated by stress-related stimuli or various intracellular microbes. GRA24 = PSP7 Mouse bone marrow-derived macrophages (BMDM) were infected with the following Toxoplasma gondii strains: - RHku80 WT versus RHku80(deltaPSP7) mutant - Pruku80 WT versus Pruku80(deltaPSP7) mutant
2013-09-01 | E-GEOD-38782 | biostudies-arrayexpress
Project description:Toxoplasma gondii is an obligate intracellular protozoan parasite that poses a severe threat to human health, especially those immune-compromised individuals. Sulfadiazine sodium (SDZ) is widely used for the treatment of human toxoplasmosis. However, systems biology researches on the therapeutic effects and mechanisms are not yet well performed. After T. gondii GT1 (Genotype I) infection, BALB/c mice were treated orally with SDZ (250 µg/mL in water) for consecutive 12 days. Mice showed typical acute toxoplasmosis symptoms on day 20 post infection, and 20 percent survived on day 30 post infection and established a chronic infection. An untargeted metabolomics approach based on an UPLC-MS/MS method was employed to identify the serum metabolic changes during SDZ treatment. Multivariate statistical analyses (PCA and PLS-DA) were employed to profile the serum metabolomes from the control mice, acute infection mice and SDZ treated mice. In total, 16,426 metabolite ions could be detected and 14,039 of them were consistently detected in more than 70% of the runs. Our results identified common and uniquely perturbed metabolites and pathways among acutely infected or SDZ treated groups. Acutely infected mice showed a dramatic global metabolic perturbations, and SDZ had a favorable therapeutic effect on parasite-induced acute toxoplasmosis by adjusting the metabolic disorders. Notably, serum metabolomics is proved to be a powerful and reliable approach into the surveillance of disease progression and treatment. More importantly, findings in this study will help provide useful data support for the development of new and safe pharmaceuticals against human toxoplasmosis.
2019-06-18 | MTBLS852 | MetaboLights