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Schmidtea mediterranea

ABSTRACT: RNA Seq analysis of sorted Schmidtea mediterranea X1 and Xins cells to identify factors specific to neoblasts

PROVIDER: PRJNA296017 | ENA |

REPOSITORIES: ENA

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			Action	DRS
	SRR2407880.fastq.gz	Fastqsanger.gz
	SRR2407881.fastq.gz	Fastqsanger.gz
	SRR2407874.fastq.gz	Fastqsanger.gz
	SRR2407875.fastq.gz	Fastqsanger.gz
	SRR2407876.fastq.gz	Fastqsanger.gz

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Transcriptome analysis reveals strain-specific and conserved stemness genes in Schmidtea mediterranea

Project description:The transcriptome of the planarian Schmidtea mediterranea is not well characterized. We have used RNA-Seq to characterize the transcriptome in both sexual and asexual strains of S. mediterranea from both untreated and irradiated animals. Moreover, we have performed RNA-Seq on RNA purified from FACS sorted neoblasts and differentiated cells. Together these studies expand our understanding of the planarian transcriptome and have identified strain-specific, neoblast-specific, and conserved transcripts. RNA-Seq was performed on RNA isolated from untreated and irradiated S. mediterranea animals from both the sexual and asexual strains, on FACS purified X1 neoblasts, X2 neoblasts, and Xins differentiated cells. One of the raw data files for GSM847465 is missing. The fasta file is provided at http://genome.vcell.uchc.edu/GenomeData02/Graveley_Lab_Public_Data/Planarian/S.mediterranea_SexNIRmRNA3.fa.gz

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