Project description:Gene expression array utilizes the advantage of the big number of the significantly changed genes creating transcriptional profiles of known mutations. Creating gene expression profiles of the animal models with known genetic defect provides great opportunity for further investigation human diseases with unknown etiology. Motheaten mice with mutation in protein tyrosine phosphatase Ptpn6 have massive neutrophilic infiltration within the skin followed by autoinflammatory and autoimmune systemic pathology resembling similarity to neutrophilic dermatoses in human. Keywords: genetic modification We performed microarray study on motheaten mice with novel mutation (meb2) in protein tyrosine phosphatase non-receptor-6 (Ptpn6) in with 5 sick bone marrow samples and 5 wild type controls and found significant difference between these two groups.
Project description:We performed scRNA in sorted LinnegcKit+ bone marrow cells from single and double Bap1 and Trp53 knockout mice to identify transcirptional programs driving erythroleukemia.
Project description:ATAC-seq profiling of Nfat5 KO and wild type macrophages derived from bone marrow (primary cells), treated or not with Lipopolysaccharide (LPS).
Project description:To describe the protein profile in hippocampus, colon and ileum tissue’ changing after the old faeces transplants, we adopted a quantitative label free proteomics approach.
Project description:We performed RNA-seq and ChIP-seq in lineage depleted bone marrow cells isolated from single and double Bap1 and Trp53 knockout mice to identify transcriptional and epigenetic programs that drive erythroleukemia.
Project description:We performed RNA-seq and ChIP-seq in lineage depleted bone marrow cells isolated from single and double Bap1 and Trp53 knockout mice to identify transcirptional and epigenetic programs that drive erythroleukemia.
Project description:In order to investigate the function of Ptpn6 in ALL, we isolated bone marrow cells from Ptpn6 +/fl mice and transformed them with BCR-ABL1. In a second transduction the BCR-ABL1 driven pre-B cells were transformed either with CRE-ERT2 or ERT2 (PMIP) and after 2 days of treatment with 4OH-Tamoxifen (0.5 micromolar) subjected to gene expression analysis.
