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Mus musculus

ABSTRACT: RNA-Seq analysis of 3 month old all Ret-positive (EGFP-labeled) dorsal root ganglia L345 or L6S1 neurons

PROVIDER: PRJNA299153 | ENA |

REPOSITORIES: ENA

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			Action	DRS
	SRR2723990.fastq.gz	Fastqsanger.gz
	SRR2724000.fastq.gz	Fastqsanger.gz
	SRR2724010.fastq.gz	Fastqsanger.gz
	SRR2723991.fastq.gz	Fastqsanger.gz
	SRR2724001.fastq.gz	Fastqsanger.gz

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Project description:RNA-SEQ analysis of postnatal Ret-positive (EGFP-labeled) nociceptive (NaV1.8 population) dorsal root ganglia L345 or L6S1 neurons.

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Project description:Nociceptors play an essential role in both acute pain and chronic pain conditions. In this study, we examined the proteome of mouse dorsal root ganglia and compared NaV1.8Cre+/-; ROSA26-flox-stop-flox-DTA (Diphtheria toxin fragment A) mutant mice (NaV1.8Cre-DTA), in which NaV1.8-positive neurons (mainly nociceptors) in dorsal root ganglia (DRG) were ablated, with respective littermate wildtype controls.

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RNA-Seq analysis of 3 month old all Ret-positive (EGFP-labeled) dorsal root ganglia L345 or L6S1 neurons

Project description:The goal of this set is to build expression data using FACS isolated Ret-positive cells harvested from a Ret-EGFP reporter mouse (3 month old). To enrich for bladder projecting neurons L6S1 DRGs were FACS isolated. L3,4, 5 lumbar levels were also FACS isolated to aid in differential expression of Ret-positive populations from L6S1 and L345 and help enrich bladder Ret-positive neurons. The series further includes these populations from males and females to uncover sex based differences.

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