Project description:This bulk RNA-seq study compares primary colorectal cancer and metastatic colorectal cancer biopsy samples, adjacent normal colorectal tissue, and human iPSC-derived intestinal organoids. The dataset supports analysis of transcriptional differences in epithelial lineage identity, inflammatory and stromal remodeling, extracellular matrix organization, metabolic adaptation, and tissue-level regulatory programs.
Project description:Tissue for normal colonic stem cell isolation was obtained via colectomy from a colorectal cancer patient. It was shown that the resected tissue and the in vitro-cultured organoids grown from tissue-derived adult stem cells do not harbor chromosomal alterations. For that purpose, genomic DNA from resected mucosa or genomic DNA from adult stem cell-derived organoids grown in a 3-dimensional culture system was compared to white blood cell genomic DNA obtained from the same individual. Furthermore, in vitro organoids which were cultured and serially passaged for several weeks did not acquire chromosomal alterations. Analysis of chromosomal stability of colonic tissue or in vitro-cultured organoids via comparison with blood cells derived from the same individual.
Project description:Patient-derived colorectal cancer organoids were injected into mice to grow into subcutaneous tumors. Tumors were profiled through multiplexed single-cell RNA-sequencing.
Project description:Tissue for normal colonic stem cell isolation was obtained via colectomy from a colorectal cancer patient. It was shown that the resected tissue and the in vitro-cultured organoids grown from tissue-derived adult stem cells do not harbor chromosomal alterations. For that purpose, genomic DNA from resected mucosa or genomic DNA from adult stem cell-derived organoids grown in a 3-dimensional culture system was compared to white blood cell genomic DNA obtained from the same individual. Furthermore, in vitro organoids which were cultured and serially passaged for several weeks did not acquire chromosomal alterations.
Project description:Patient-derived colorectal cancer organoids were injected into mice to grow into subcutaneous tumors. Starting at 100 mm3, tumors were treated with Trodelvy (Sacituzumab Govitecan (SG)) or vehicle twice per week for 28 days before samples were harvested and subjected to single-cell RNA-sequencing of the tumor cells.
Project description:We performed a comprehensive gene expression analysis of human colorectal cancer (CRC) tissues, matched adjacent normal mucosa, established CRC cell lines, and primary cultures derived from patient tumors (2D organoids). This dataset aims to identify genes and signaling pathways preferentially expressed in cancer cells and to explore clinically relevant mechanisms through comparative analysis of primary cultures and patient tissues.
