Homo sapiens
Ontology highlight
ABSTRACT:
PROVIDER: PRJNA304637 | ENA |
REPOSITORIES: ENA
Similar Datasets
Project description:The goal of this study was to analyze global gene expression in specific populations of nociceptor sensory neurons, the neurons that detect damaging/noxious stimuli. The dorsal root ganglia (DRG), trigeminal ganglia, and nodose ganglia are anatomically distinct peripheral sensory ganglia that contain nociceptors which innervate skin, gut, lungs, and other distinct organ tissues. We used flow cytometry to purify nociceptors from these ganglia and profiled their global gene expression signatures to compare gene expression between these different anatomically distinct nociceptors. Nav1.8-Cre were bred with Rosa26-TdTomato to generate Nav1.8-Cre/R26-TdTomato reporter progeny, where all peripheral nociceptor neurons are genetically marked with red fluroescence due to specific expression of the TTX- resistant sodium channel Nav1.8. Lumbar region dorsal root ganglia (DRG), trigeminal ganglia, and nodose ganglia were dissected from mice (3 mice were pooled/sample). Highly red fluorescent neurons were Facs purified, RNA extracted, and processed for microarray analysis.
2013-08-28 | E-GEOD-46546 | biostudies-arrayexpress
Project description:Nociceptors play an essential role in both acute pain and chronic pain conditions. In this study, we examined the proteome of mouse dorsal root ganglia and compared NaV1.8Cre+/-; ROSA26-flox-stop-flox-DTA (Diphtheria toxin fragment A) mutant mice (NaV1.8Cre-DTA), in which NaV1.8-positive neurons (mainly nociceptors) in dorsal root ganglia (DRG) were ablated, with respective littermate wildtype controls.
2022-10-03 | PXD034447 | Pride
Project description:Vagal afferent neurons are thought to convey primarily physiological information, whereas spinal afferents transmit noxious signals from the viscera to the central nervous system. In order to elucidate molecular identities for these different properties, we compared gene expression profiles of neurons located in nodose ganglia (NG) and dorsal root ganglia (DRG) in mice. Intraperitoneal administration of Alexa Fluor-488 conjugated Cholera toxin B allowed identification of neurons projecting to the viscera. Fluorescent neurons in DRG (from T10 to T13) and NG were isolated using laser capture microdissection. Gene expression profiles of visceral afferent neurons, obtained by microarray hybridization, were analysed using multivariate spectral map analysis, SAM algorithm (Significance Analysis of Microarray data) and fold-difference filtering. A total of 1996 genes were found to be differentially expressed in DRG versus NG, including 41 G-protein coupled receptors and 60 ion channels. Expression profiles obtained on laser-captured neurons were contrasted to those obtained on whole ganglia demonstrating striking differences and the need for microdissection when studying visceral sensory neurons because of dilution of the signal by somatic sensory neurons. Furthermore, a detailed catalogue of all adrenergic and cholinergic, GABA, glutamate, serotonin and dopamine receptors, voltage-gated potassium, sodium and calcium channels and transient receptor potential cation channels present in visceral afferents is provided. Our genome-wide expression profiling data provide novel insight into molecular signatures that underlie both functional differences and similarities between NG and DRG visceral sensory neurons. Moreover, these findings will offer novel insight into mode of action of pharmacologic agents modulating visceral sensation. Experiment Overall Design: Three separate experiments were performed. First, 5 whole dorsal root ganglia were compared to 7 whole nodose ganglia. Second, Laser captured visceral neurons derived from 5 dorsal root ganglia and 5 nodose ganglia were compared on MG-U74Av2. Third, Laser captured visceral neurons derived from 9 dorsal root ganglia and 11 nodose ganglia were compared on Mouse430_2.
2007-08-31 | E-GEOD-2917 | biostudies-arrayexpress
Project description:The peripheral nervous system (PNS) orchestrates organ function during homeostasis and stress. Most cancers including pancreatic ductal adenocarcinoma (PDAC) are infiltrated by PNS neurons participating in their complex tumor microenvironment. Here, we analyze the effect of neuronal interactions with fibroblasts and cancer cells with dorsal root and celiac ganglia. To this end, both cell types were co-cultured with both peripheral ganglia and subjected to bulk RNA-Sequencing.
2025-02-28 | E-MTAB-12899 | biostudies-arrayexpress
Project description:The goal of this study was to analyze global gene expression in specific populations of nociceptor sensory neurons, the neurons that detect damaging/noxious stimuli. The dorsal root ganglia (DRG), trigeminal ganglia, and nodose ganglia are anatomically distinct peripheral sensory ganglia that contain nociceptors which innervate skin, gut, lungs, and other distinct organ tissues. We used flow cytometry to purify nociceptors from these ganglia and profiled their global gene expression signatures to compare gene expression between these different anatomically distinct nociceptors.
2013-08-28 | GSE46546 | GEO
Project description:We applied TurboID-based proximity labelling restricted to Advillin-expressing neurons to systematically profile the proteomes of distinct neuronal subcompartments, using parallel accumulation–serial fragmentation combined with data-independent acquisition (diaPASEF) mass spectrometry. Neuronal compartments include somata (L3–L5 dorsal root ganglia, DRG), presynaptic terminals (dorsal lumbar spinal cord; dLSC), axons (sciatic nerve; SCN), and peripheral terminals (paw skin), providing the first proteomic atlas derived of/enriched in lumbar sensory neurons. Our findings provide novel insights into the compartment-specific protein distributions that underlie key aspects of sensory neuron function. To highlight to effectiveness of this approach. we also explored the neuronal proteome on DRG explants exposed to Oxaliplatin (or Vehicle) to extract neuron-specific changes upon chemotherapy exposure. In both cases, the corresponding, wildtype (whole tissue) proteomes were also profiled with diaPASEF. Together, these data serve as a valuable resource for future studies on neuronal specialization and pain-related proteomic alterations in the PNS.
2026-04-14 | PXD062198 | Pride
Project description:Transcription profiling of Ret-positive subpopulations of Dorsal Root Ganglia neurons in mouse
2016-08-01 | E-MTAB-1836 | biostudies-arrayexpress
Project description:Here we studied the NOX2 dependent redox-proteome in dorsal root ganglia in mice. The overall goal was to assess the degree of NOX2-dependent changes in oxidised proteins following exposure to enriched enviroment and sciatic nerve axotomy in dorsal root ganglia.
2020-10-13 | PXD018085 | Pride
Project description:Mycolactone is a mycobacteria-derived macrolide that blocks the biogenesis of a large array of secreted and transmembrane proteins through potent inhibition of the Sec61 translocon. Here, we used quantitative proteomics to delineate the direct and indirect effects of mycolactone-mediated Sec61 blockade on mouse on MED17.11 cells, as a model of dorsal root ganglia (DRG) sensory neurons, in resting and LPS-stimulated conditions. This analysis completes two previously reported ones, which investigated the effects of mycolactone on the proteome of the mouse MutuDC dendritic cells (Project PXD006103) and human Jurkat T cells (Project PXD002971).
2018-06-26 | PXD007770 | Pride
Project description:Loss of Prdm12 induces gene deregulation in mature dorsal root ganglia neurons of adult mice
2022-01-26 | GSE182901 | GEO