Project description:ASCL1 is known to act as transcriptional activator of notch signaling pathway. We have found that ASCL1 is over expressed in secondary glioblastoma. In order to delineate its functional role in gliomagensis we have used gene silencing approach to identify genes differentially regulated upon ASCL1 down regulation in U87MG glioma cell line.
Project description:This experiment is designed to evaluate gene expression alteration following AEG-1 (MTDH) silencing in glioma cells. AEG-1 is found to be a potential regulator through interaction with other transcription factor. We intended to investigate the enriched transcription factor dependent downstream gene sets regulated by AEG-1 form the differential expressed genes we obtained from AEG-1 silencing. Total RNA were extracted from U87MG glioma cells stably silencing AEG-1 and correspondent pSuper Retro vector cells respectively. Passage 5 of the indicated cells after infection and selection were harvested for RNA extraction.
Project description:BRD9 is a glioma-associated epigenetic regulator. This study profiles transcriptomic and chromatin-level changes in glioma cells after I-BRD9 treatment. RNA-seq was performed in U87MG and SKMG-1 cells, and ATAC-seq and BRD9 CUT&Tag were performed in U87MG cells. These datasets were used to characterize changes in gene expression, chromatin accessibility, and BRD9-associated chromatin signals after BRD9 inhibition.
Project description:Gene expression profiling of immortalized human mesenchymal stem cells with hTERT/E6/E7 transfected MSCs. hTERT may change gene expression in MSCs. Goal was to determine the gene expressions of immortalized MSCs.
Project description:Phosphatase EYA1 was overexpressed in glioma cell line U87MG and phospho-proteomic analysis was performed to identify novel EYA1 substrates in glioma cells.
