Project description:The similarity of Lyme borreliosis to other diseases and the complex pathogenesis cause diagnostic and therapeutic difficulties. Changes at the cellular and molecular level after Borrelia sp. infection remain still poorly understood. Therefore, the present study focused on the gene expression in human dermal fibroblasts in differentiation of infection with Borrelia garinii, Borrelia afzelii and Borrelia burgdorferi sensu stricto spirochetes. For microarray analysis 10 samples were used: 3 control samples - K, 2 samples of NHDF cells infected with Borrelia garinii - G, 2 samples of NHDF cells infected with Borrelia afzelii - A and 3 samples of NHDF cells infected with Borrelia burgdorferi sensu stricto - SS.
Project description:The aim of the study was to compare the global transcriptional responses elicited in NHDF cells by three different strains of Borrelia burgdorferi ss (the agent of Lyme borreliosis), representative of different stages in the life cycle of Borrelia: one reference strain isolated from a tick (strain N40), and two invasive strains isolated from skin biopsy of erythema migrans (strain Pbre c4) and acrodermatitis chronica atrophians skin lesions (strain 1408 c1). Three different experimental conditions have been tested: (1) unstimulated NHDF vs NHDF stimulated by Borrelia strain N40 / (2) unstimulated NHDF vs NHDF stimulated by Borrelia strain Pbre c4 / (3)M-BM- unstimulated NHDF vs NHDF stimulated by Borrelia strain 1408 c1. There is 2 biological replicates for each condition. All NHDF stimulation have been performed in independent experiments.
Project description:Transcriptional profiling of NHDF Cells comparing control untreated fibroblasts with fibroblasts coincubated with three different species of the Borrelia burgdorferi sensu lato group.
Project description:To reveal the comprehensive gene expression of normal human dermal fibroblasts (NHDFs) based on the interaction of GlcNAc-bearing polymer, AC-GlcNAc10, with cell surface vimentin at 24 h, we performed a DNA microarray for NHDFs treated with AC-GlcNAc10. The DNA microarray analysis revealed altered expression of approximately 1000 genes in NHDF vs. NHDF treated with AC-GlcNAc10 by extracting differentially expressed genes with the ratio of >1.5 and <0.66-fold changes between the gene expressions of NHDF vs. NHDF treated with AC-GlcNAc10, and Z-score >2.0 and <-2.0. We observed the downregulation of gene expression related to cell cycle progression, such as baculoviral IAP repeat-containing protein 5 (BIRC5; survivin), and the upregulation of gene expression related to cell cycle arrest, such as cyclin-dependent kinase inhibitor 1A (CDKN1A; p21).
Project description:We have used RNA-sequencing on six different proliferating cell lines consisting of normal human dermal fibroblasts (NHDF), normal human epidermal keratinocytes (NHEK), pericytes (PC), human microvADSCular blood endothelial cells (HMEC), lymphatic endothelial cells (LEC) and adipose derived stem cells (ADSC), subjected to different doses of radiotherapy.
Project description:Human umbilical vein endothelial cells (HUVECs) formed capillary structures when co-cultured with normal human dermal fibroblasts (NHDFs). HUVEC competence and NHDF supportiveness of cord formation were found to be highly cell-passage dependent with the early passage cells forming more angiogenic cord structures. We thus profiled gene expression in NHDFs with different passages to understand the molecular mechanisms underlying the in vitro angiogenesis control. Keywords: Time course
Project description:Normal human dermal fibroblasts (NHDF) were treated under serum-free conditions with cell culture media conditioned by breast cancer cell lines (SkBr3, MDA-MB-468, T47D) for 72 hours and subjected to gene expression profiling with Illumina platform.
Project description:We applied direct conversion of normal human dermal fibroblast (NHDF) into adipocyte-like cells using adipogenic cocktail containing IBMX, dexamethasone, insulin, and rosiglitazone and confirmed prominent lipid droplet accumulation in the converted cells for proteomic research
Project description:Normal human dermal fibroblasts (NHDF) and human lung microvascular endothelial cells (HMVEC-L) were irradiated with protons (0, 0.5, 1 and 2 Gy, 1GEv/n) at Brookhaven National Labs (BNL). Aim of the study is to find differentially transcribed genes in dependence of radiation dose/source and cell type.
