Project description:Genome-wide assessment of gene expression in primary acute lymphoblastic leukemia cells was performed to identify genomic determinants of MTXâs antileukemic effects. Reduction of circulating leukemia cells after in vivo methotrexate treatment served as a measure MTX's antileukemic effects. Experiment Overall Design: Gene expression in diagnostic primary acute lymphoblastic leukemia cells from bone marrow of 161 pediatric patients
Project description:MicroRNA-sequencing of the bone marrow samples from Brazilian pediatric patients with B-cell acute lymphoblastic leukemia (B-ALL) and T-cell acute lymphoblastic leukemia (T-ALL).
Project description:Early T-cell precursor acute lymphoblastic leukemia (ETP-ALL) develops from very early cells with the potential for both T-cell and myeloid differentiation. The ambiguous nature of leukemic blasts in ETP-ALL may lead to immunophenotypic alterations at relapse. Here, we address immunophenotypic alterations and related classification issues, as well as genetic features of relapsed pediatric ETP-ALL. RNAseq was performed with the primary goal of crypric gene fusions search within subcohorts of relapsed and non-relapsed patients with sufficient material available. Overall, 3 relapsed and 8 non-relapsed patients were tested. As a relsult, 1 relasped and 3 non-relapsed patients were found to carry leukemia-specific fusions, of those 3 were confurmed by FISH (2 cases of ETV6 rearrangements and one PICALM::MLLT10-positive case) while only one cryptic rearrangement was caught in a non-relapsed patient (BCL11B cryptic rearrangement negative by FISH). Other 7 patients were not found to carry any leukemia-specisic fusions.
Project description:Proteogenomic analysis and genomic profiling, RNA-sequencing, and mass spectrometry-based analysis of High hyperdiploid childhood acute lymphoblastic leukemia.
