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Drosophila melanogaster

ABSTRACT: Identification of transcripts whose abundance changes in response to acute switches in DSX isoform in the ovary and testis

PROVIDER: PRJNA315422 | ENA |

REPOSITORIES: ENA

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			Action	DRS
	SRR3232010.fastq.gz	Fastqsanger.gz
	SRR3232020.fastq.gz	Fastqsanger.gz
	SRR3232030.fastq.gz	Fastqsanger.gz
	SRR3232011.fastq.gz	Fastqsanger.gz
	SRR3232021.fastq.gz	Fastqsanger.gz

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Drosophila melanogaster

Project description:Identification of transcripts whose abundance changes in response to acute switches in DSX isoform

| PRJNA241132 | ENA

Identification of transcripts whose abundance changes in response to acute switches in DSX isoform in the ovary and testis

Project description:RNAseq of dissected adult ovary and testis after DSX isoform is switched using temperature inducible alleles or constructs.

2016-03-17 | GSE79294 | GEO

Identification of transcripts whose abundance changes in response to acute switches in DSX isoform in the ovary and testis

Project description:RNAseq of dissected adult ovary and testis after DSX isoform is switched using temperature inducible alleles or constructs. Biological duplicates were sequenced for each genotype at each condition.

2016-03-17 | E-GEOD-79294 | biostudies-arrayexpress

Drosophila Sex Hierarchy Regulated Gene Expression in 48 hour APF Pupae

Project description:Drosophila melanogaster undergoes a complete metamorphosis, during which time the larval male and female forms transition into sexually dimorphic, reproductive adult forms. To understand this complex morphogenetic process at a molecular-genetic level, we performed whole genome microarray analyses. In somatic tissues at 48 hour After Puparium Formation (APF), 173 sex-biased transcripts that likely function downstream of the doublesex (dsx) branch of the sex determination hierarchy were identified. The mode of regulation of the sex-specific isoforms of DSX (DSX-F and DSX-M) was examined. It was determined that for most downstream targets, DSX-F and DSX-M regulate gene expression in the same manner, but that one isoform acts as a more potent regulator. Keywords: wild type; genetic modification All microarrays were dual channel with direct comparisons of male versus female or wild type versus mutant. All samples consist of whole body pupae collected at 48 hour After Puparium Formation (APF). For each experiment, four biological replicates were analyzed in a dye-swap design.

2009-02-10 | E-GEOD-11311 | biostudies-arrayexpress

Drosophila Sex Hierarchy Regulated Gene Expression in 48 hour APF Pupae

2009-02-11 | GSE11311 | GEO

PolyA+ RNA-Seq profiling of Drosophila melanogaster late third instar larval ovary, testis and sex transformed gonads as a result of tra and dsx perturbation.

Project description:We designed this experiment to investigate the transcriptional changes in gonads as a result of sex transformation. Here we performed transcriptional profiling of the ovary transformed into testis from the tra loss of function (XX_tra_lof), testis transformed into ovary from the tra gain of function (XY_tra_gof) and ovary transformed into testis in dsxM gain of function (XX_DsxM_gof/lof) Drosophila melanogaster third instar larvae in biological quadruplicates. In addition, as controls we sequenced ovaries and testes from the female and male wildtype larvae respectively. We constructed polyA+ libraries of the gonads, cleaned off the fatbody and performed 50 bp, stranded single-end RNA-Seq.

2022-06-16 | GSE205406 | GEO

Identification of transcripts whose abundance changes in response to acute switches in DSX isoform

Project description:RNAseq of dissected adult fat body after DSX isoform is switched using temperature inducible alleles or constructs. Biological duplicates were sequenced for each genotype at each condition.

2014-10-30 | E-GEOD-55848 | biostudies-arrayexpress

Identification of transcripts whose abundance changes in response to acute switches in DSX isoform

Project description:RNAseq of dissected adult fat body after DSX isoform is switched using temperature inducible alleles or constructs.

2014-10-30 | GSE55848 | GEO

Regulation of the gonadal transcriptome during sex determination and testis morphogenesis: comparative candidate genes

Project description:Embryonic day 13 (E13), E14, and E16 rat testes and ovaries were used for microarray analysis, as well as E13 testis organ cultures that undergo testis morphogenesis and develop seminiferous cords in vitro. A list of 109 genes resulted from a selective analysis for genes present in male gonadal development and with a 1.5-fold change in expression between E13 and E16. Characterization of these 109 genes potentially important for testis development revealed that cytoskeletal-associated proteins, extracellular matrix factors, and signaling factors were highly represented. Throughout the developmental period (E13-E16), sex-enriched transcripts were more prevalent in the male with 34 of the 109 genes having testis-enriched expression during sex determination. In ovaries, the total number of transcripts with a 1.5-fold change in expression between E13 and E16 was similar to the testis, but none of those genes were both ovary enriched and regulated during the developmental period. Genes conserved in sex determination were identified by comparing changing transcripts in the rat analysis herein, to transcripts altered in previously published mouse studies of gonadal sex determination. A comparison of changing mouse and rat transcripts identified 43 genes with species conservation in sex determination and testis development. Profiles of gene expression during E13-E16 rat testis and ovary development are presented and candidate genes for involvement in sex determination and testis differentiation are identified. Analysis of cellular pathways did not reveal any specific pathways involving multiple candidate genes. However, the genes and gene network identified influence numerous cellular processes with cellular differentiation, proliferation, focal contact, RNA localization, and development being predominant. Keywords: expression analysis, testis, ovary, sex determination

2008-02-20 | GSE10558 | GEO

Pervasive 3'-UTR isoform switches during mouse oocyte maturation

Project description:Oocyte maturation is the foundation for developing healthy individuals of mammals. Upon germinal vesicle breakdown, oocyte meiosis resumes and the synthesis of new transcripts ceases. To quantitatively profile the transcriptomic dynamics after meiotic resumption throughout the oocyte maturation, we generated transcriptome sequencing data with individual mouse oocytes at three main developmental stages: germinal vesicle (GV), metaphase I (MI), and metaphase II (MII). When clustering the sequenced oocytes, results showed that isoform-level expression analysis outperformed gene-level analysis, indicating isoform expression provided extra information that was useful in distinguishing oocyte stages. Comparing transcriptomes of the oocytes at the GV stage and the MII stage, in addition to identification of differentially expressed genes (DEGs), we detected many differentially expressed transcripts (DETs), some of which came from genes that were not identified as DEGs. When breaking down the isoform-level changes into alternative RNA processing events, we found the main source of isoform composition changes was the alternative usage of polyadenylation sites. With detailed analysis focusing on the alternative usage of 3'-UTR isoforms, we identified, out of 3810 tested genes, 512 (13.7%) exhibiting significant switches of 3'-UTR isoforms during the process of moues oocyte maturation. Altogether, our data and analyses suggest the importance of examining isoform abundance changes during oocyte maturation, and further investigation of the pervasive 3'-UTR isoform switches in the transition may deepen our understanding on the molecular mechanisms underlying mammalian early development.

2021-10-05 | GSE178836 | GEO

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