Project description:To explore the differences in miRNA profiles, naive CD4+CD62L+ helper T cells were sorted by magnetic cell sorting from spleens of female C57BL/6 mice and were induced to differentiate into Th1 or Th17 cells, then total RNA was extracted and miRNA-seq were conducted. The results showed that many microTNAs presented a different expression pattern between Th1 and Th17 cells, which prompted us to further study their roles in Th117 differenciation. miRNA profiles of Th0 and Th17 cells were generated by deep sequencing, in triplicate, using Illumina HiSeq 2500
Project description:Purpose: RNA-seq analysis of gene expression patterns in Naïve t, Th0, Treg and Th17 cells of wild-type mice. The goals of this study are to compared the gene expression between Naïve T and other types Th cells by RNA-seq analysise.
Project description:Purpose: RNA-seq analysis of gene expression patterns in Naïve t, Th0, Treg and Th17 cells of wild-type mice. The goals of this study are to compared the gene expression between Naïve T and other types Th cells by RNA-seq analysise.
Project description:To investigate the transcriptomic changes of Th0 or Th17 cells by IPMK, naïve CD4 T cells of Ctrl and IPMK-KO mice were sorted by FACS and differentiated into Th0 or Th17 cells and RNA seq was performed.
Project description:The aim of the study was to investigate whether the trefoil peptide genes, in concerted action with a miRNA regulatory network, were contributing to nutritional maintrenance. Using a Tff3 knock-out mouse model, 21 specific miRNAs were noted to be significantly deregulated when compared to the wild type strain.
Project description:The aim of the study was to investigate whether the trefoil peptide genes, in concerted action with a miRNA regulatory network, were contributing to nutritional maintrenance. Using a Tff2 knock-out mouse model, 48 specific miRNAs were noted to be significantly deregulated when compared to the wild type strain.
Project description:T cells from the spleen of a mixed-gender pool of four double-transgenic 2D2 (TCRMOG) x IgHMOG mice (OSE) mice, which spontaneously develop experimental autoimmune encephalomyelitis (EAE), were used to derive TH1- and TH17-polarized cells, as described in https://doi.org/10.1371/journal.pone.0015531. Here, expression was compared, first, between TH1 and naïve TH0 and, second, between TH17 and naïve TH0 cells.