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Bacillus subtilis subsp. subtilis str. 168

ABSTRACT: Analysis of differential gene expression during Bacillus subtilis spore outgrowth in high-salinity environments using RNA sequencing

PROVIDER: PRJNA321031 | ENA |

REPOSITORIES: ENA

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			Action	DRS
	SRR3488630.fastq.gz	Fastqsanger.gz
	SRR3488631.fastq.gz	Fastqsanger.gz
	SRR3488622.fastq.gz	Fastqsanger.gz
	SRR3488632.fastq.gz	Fastqsanger.gz
	SRR3488623.fastq.gz	Fastqsanger.gz

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Whole proteome analysis of germinating Bacillus subtilis 168

Project description:In this work we present proteomic analysis of Bacillus subtilis 168 trp+ (BSB1) during germination and spore outgrowth. Samples were collected from 14 different time points (from 0 to130 minutes) after inoculation of spores into germination medium. In summary, 2,195 proteins were identified and clustered according to their expression kinetics. Results revealed five specific clusters that were functionally analyzed.

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Bacillus subtilis subsp. subtilis str. 168

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Project description:Characterization of the putative genetic determinants of the VBNC state in a known spore-forming Gram-positive organism Bacillus subtilis 168. The VBNC state was induced under osmotic stress and aminoglycoside treatment. The transcriptome landscape of VBNC cells was compared to the viable, antibiotic sensitive B. subtilis cells and to the viable cells with no antibiotic treatment.

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Project description:To gain insight into spore germination and outgrowth, the transcriptome changes during Bacillus subtilis spore conversion to vegetative cells were analyzed. The transcriptome analysis also allowed us to trace the different functional groups of genes expressed during this conversion. . Our analysis identified 34 abundant mRNA transcripts in the dormant spores, at least 31 of which were rapidly degraded after the phase transition and observed 3152 differentially expressed genes during spore germination and outgrowth.

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RNA-seq established gene expression data to determine the effects of various stresses on Bacillus subtilis str.168

Project description:To explore the effects of different stress conditions on Bacillus subtilis str.168, a selection of conditions were applied to the organism and RNA-seq data gathered. A matrix of gene counts was produced as a basis for further analysis into the transcription profiles of Bacillus subtilis str.168.

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Bacillus subtilis WalR (YycF) ChIP-chip Bacillus subtilis; Bacillus subtilis subsp. subtilis str. 168

Project description:Identification of the specific WalR (YycF) binding regions on the B. subtilis chromosome during exponential and phosphate starvation growth phases. The data serves to extend the WalRK regulon in Bacillus subtilis and its role in cell wall metabolism, as well as implying a role in several other cellular processes. For each sample analyzed in this study three biological replicates were performed. Three different samples were taken from a strain expressing the WalR-SPA protein as well as from wild-type (168) without a tagged WalR. Samples were taken from exponentially growing cells in low phosphate medium (LPDM) as well as from phosphate-limited cells (T2). Each sample compares ChIP DNA vs. Total DNA from the same cells.

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Expression data from Bacillus subtilis 168 during growth

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