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Saccharomyces cerevisiae

ABSTRACT: Role of 14-3-3 in the transcriptional response of Saccharomyces cerevisiae to potassium starvation.

PROVIDER: PRJNA338803 | ENA |

REPOSITORIES: ENA

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			Action	DRS
	SRR4026590.fastq.gz	Fastqsanger.gz
	SRR4026591.fastq.gz	Fastqsanger.gz
	SRR4026592.fastq.gz	Fastqsanger.gz
	SRR4026593.fastq.gz	Fastqsanger.gz
	SRR4026594.fastq.gz	Fastqsanger.gz

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Saccharomyces cerevisiae

Project description:Transcriptional response of Saccharomyces cerevisiae to potassium starvation

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Transcriptional response of Saccharomyces cerevisiae to potassium starvation

Project description:In this study we investigated the transcriptional response of the yeast Saccharomyces cerevisiae to potassium starvation. To this end yeast cells were grown for 60 min in media without potassium or in media with a standard potassium concnetration (50 mM KCl). Using Serial Analysis of Gene Expression (SAGE)-tag sequencing the effect of potassium starvation on the transcriptome was determined.

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Role of 14-3-3 in the transcriptional response of Saccharomyces cerevisiae to potassium starvation.

Project description:We investigated the transcriptional response of yeast Saccharomyces cerevisiae bmh1 and bmh2 deletion mutants to potassium starvation. To this end yeast strains were grown for 60 min in media without potassium or in media with a standard potassium concentration (50 mM KCl). Using Serial Analysis of Gene Expression (SAGE)-tag sequencing the effect of potassium starvation on the transcriptome was determined. This study is a follow-up of our previous study (Anemaet IG and van Heusden GPH. 2014. BMC Genomics:1040)( GEO accession number GSE57093).

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Project description:Metabolite concentrations can regulate gene expression, which can in turn regulate metabolic activity. The extent to which functionally related transcripts and metabolites show similar patterns of concentration changes, however, remains unestablished. We have therefore measured and analyzed the metabolomic (previously published in Brauer et al., PMID 17159141) and transcriptional responses (presented here) of Saccharomyces cerevisiae to carbon and nitrogen starvation.

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Project description:Tda1 in Saccharomyces cerevisiae is a protein kinase that is activated in response to glucose starvation. However, how it is activated has not been investigated for a long time. In this analysis, yeast cells were grown in high (2%) or low (0.05%) glucose medium, and the lysate were subjected to immunoprecipitation of Tda1-3xHA with an anti HA antibody. Proteins in the immnoprecipitated fractions were digested and peptides were analyzed by MS/MS.

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