Ontology highlight
ABSTRACT:
PROVIDER: PRJNA350159 | ENA |
REPOSITORIES: ENA
Similar Datasets
Project description:•Carnivorous plants from the order Caryophyllales co-opted plant phytohormones from the group of jasmonates for regulation of digestive enzyme activities. But not all genera of carnivorous plants are evenly explored and the digestive physiology of the Australian carnivorous rainbow plants of the genus Byblis (order: Lamiales) is only very poorly understood. •Here, we investigated the composition of digestive enzymes in the secreted fluid of Byblis filifolia using LC/MS, measured enzyme activities and analysed the phytohormone tissue level after experimental feeding on fruit flies and coronatine application. •Several hydrolytic digestive enzymes were identified in the secreted digestive fluid which were clearly upregulated in the presence of insect prey. But in contrast to sundew plant Drosera capensis, endogenous jasmonate did not accumulate and coronatine, a molecular mimic of jasmonates, was not able to trigger enzyme secretion. •Our results document that carnivorous plant B. filifolia is fully carnivorous with their own digestive enzyme repertoire. However in contrast to carnivorous genera from the order Caryophyllales, they are not under the control of jasmonates. This indicates that jasmonates have not been co-opted for regulation of digestive enzyme activity repeatedly during evolution of carnivorous plants.
2025-05-20 | PXD060459 | Pride
Project description:Fungal endophytes of carnivorous and non-carnivorous bog plants
| PRJNA1095839 | ENA
Project description:Charles Darwin was infinitely fascinated with Drosera (sundew plants), which have behaved as the most sagacious animals and evolved as flesh-eaters with modified leaves, called traps, to catch and digest insects. However, the molecular basis underlying movement of carnivorous plants remains very limited. Here, we uncover glutathione (GSH) as a signaling molecule in sundew plants that induces trap movement for carnivory. To better understanding the molecular basis of sundew carnivory, we further performed transcriptomic profiling of closed sundew traps stimulated by living fruit flies and GSH.
2026-04-21 | GSE249815 | GEO
Project description:Gene expression evolution in convergently evolved carnivorous plants
| PRJDB15737 | ENA
Project description:The marsh pitcher plant (Heliamphora sp.) is a genus of carnivorous plant in which the production of endogenous digestive enzymes is still dubious. In this study, we tried to elucidate these old doubts and we investigated the ability of prey digestion in H. parva, H. neblinae and hybrid H. heterodoxa x minor. The plants were fed on fruit flies and digestive fluids were analysed using LC/MS, Western blots and enzyme activity measurements for presence of endogenous digestive enzymes. All investigated species produced endogenous digestive enzymes similar to those found in other carnivorous plant genera and the sister genus Sarracenia. Among these are: aspartic protease, subtilisin-like protease, type III chitinase, beta-1,3-glucanase, glucosidase, GDSL lipase, thaumatin-like protein etc. Phosphatase and proteolytic activities increased over time irrespective of feeding status. Immunoblotting of aspartic protease showed that abundance of the enzyme in digestive fluid can accumulate over time without any prey stimuli. The species of the genus Heliamphora can be considered as truly carnivorous plants with ability to produce endogenous digestive enzymes, although these are probably strongly diluted by rain in natural habitat, questioning their ecophysiological significance.
2026-08-03 | PXD063061 | Pride
Project description:Of the members of mitochondrial transcription termination factors (mTERFs) found in metazoans and plants known to regulate organellar gene expression at various levels, plant mTERF6 promotes maturation of a tRNA We used microarrays to analyze whole-genome transcriptome changes in pam48 (mterf6-1) mutants compared to wild type.
2015-12-10 | GSE75824 | GEO
Project description:Transcriptional profiling of Arabidopsis thaliana 12-days old seedlings comparing Col-0 wild type with transgenic plants with altered expression of dual-targetting plastid/mitochondrial organellar RNA-polymerase RPOTmp. Transgenic plants used for experiment were: overexpressor plants obtained by transformation of Col-0 WT plants with genetic constructs created in [Tarasenko et al., 2016] contained catalytic part of RPOTmp enzyme with transit peptides of RPOTm (mitochondrial) and RPOTp (plastid) by agrobacterial transformation; plants with complementation of RPOTmp functions in mitochondria or chloroplasts obtained from transformation of GABI_286E07 rpotmp knockout-mutant plants with genetic constructs created in [Tarasenko et al., 2016]. Goal was to determine the effects of RPOTmp knockout/overexpression on global Arabidopsis thaliana gene expression.
2023-12-25 | GSE251661 | GEO
Project description:Pentatricopeptide repeat (PPR) proteins, which are characterized by tandem 30-40 amino acid sequence motifs, constitute a large gene family in plants. These known PPR proteins have been identified to play important roles in organellar RNA metabolism and plant development in Arabidopsis and rice. However, functions of PPR genes in woody species remain still largely unknown. Here, we identified and characterized a total of 626 PPR genes containing PPR motifs in the poplar genome. A comprehensive genome-wide analysis of the poplar PPR gene family was performed, including chromosomal location, phylogenetic relationships, gene duplication. Transcriptomic analyses identified that 154 of the PtrPPR genes were induced by biotic and abiotic treatments, including Marssonina brunnea, salicylic acid (SA), methyl jasmonate (MeJA), wounding, cold and salinity. Quantitative RT-PCR analysis further confirmed the expression profiles of 11 PtrPPR genes under different stresses. Our results contribute to a more comprehensive understanding the roles of PPR proteins and provided an insight for improving the stress tolerance in poplar.
2018-01-31 | GSE109609 | GEO