Project description:Schistosoma mansoni is the causative agent of schistosomiasis, an endemic neglected tropical disease that affects the human population. Recently, newer versions of the genome and transcriptome have been published, with the Sanger Institute leading both the original publication of the genome as well as the further upgrade. However, little is known about the regulatory elements found in the genome, i.e. promoters. Identification of these elements is key for the understanding of mechanisms of regulation of gene expression in this parasitic helminth.
2014-08-20 | E-ERAD-96 | biostudies-arrayexpress
Project description:Population genomics-guided engineering of phenazine biosynthesis in Pseudomonas chlororaphis
Project description:Purpose: To determine effects of arsenic on gene expression in polarized primary human bronchial epithelial (HBE) cells and impact on transcriptional response to Pseudomonas aeruginosa infection Methods: mRNA profiles of HBE cells from 6 donors exposed to 0, 5, 10 or 50 ug/L total arsenic +/- Pseudomonas aeruginosa (48 samples) were generated using Illumina sequencing, aligned in CLC Genomics workbench and analyzed for DE in EdgeR Findings: 20-30 million reads were mapped per sample and transcripts were identifed that were significantly differentially expressed in response to arsenic and Pseudomonas aeruginosa
Project description:Single-cell RNA sequencing (scRNA-seq) was performed on virgin/adult females using 10x Genomics to explore transcriptome change after BATT induction. Egg production by female Schistosoma is central to both the transmission and pathology of schistosomiasis, a neglected tropical disease affecting over 200 million people globally. Female sexual maturation depends on the male-derived pheromone β-alanyl-tryptamine (BATT), yet the molecular mechanisms underlying this process remain largely unknown.
