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ABSTRACT: Wood and straw culture substrate biodegraded molecular mechanism of Pleurotus ostreatus through Transcriptome Sequencing technology

PROVIDER: PRJNA387513 | ENA |

REPOSITORIES: ENA

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			Action	DRS
	SRR5585710_1.fastq.gz	Fastqsanger.gz
	SRR5585710_2.fastq.gz	Fastqsanger.gz
	SRR5585711_1.fastq.gz	Fastqsanger.gz
	SRR5585711_2.fastq.gz	Fastqsanger.gz
	SRR5585703_1.fastq.gz	Fastqsanger.gz

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Pleurotus ostreatus

Project description:Wood and straw culture substrate biodegraded molecular mechanism of Pleurotus ostreatus through Transcriptome Sequencing technology

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Gene age predicts the transcriptional landscape of sexual morphogenesis in multicellular fungi [Pleos]

Project description:Pleurotus ostreatus tissue types and developmental stages

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Impact of transposable elements in Pleurotus ostreatus transcriptome profiles

Project description:Pleurotus ostreatus, also known as the oyster mushroom, is an active lignin decomposer in the forests. The genomes of the monokaryotic strains PC15 and PC9 have been used to characterize the content and distribution of transposable elements. This study analyzes the impact of transposable element insertions on the global transcriptome of P. ostreatus. The transcriptome of PC15 and PC9 has been analyzed in exponential growth during submerged fermentation in malt-yeast extract-sucrose medium RNAseq of two P. ostreatus strains: PC15 and PC9

2016-05-19 | E-GEOD-81586 | biostudies-arrayexpress

LC-MS/MS secretome analysis of Pleurotus ostreatus PC9 (wild type) and two transformants

Project description:The white rot fungi Pleurotus ostreatus was studied for its potential as a biological pretreatment agent, i.e- its potential for decomposing lignocellulosic biomass. P. ostreatus PC9 was manipulate to either overexpress or eliminate (by gene replacement) the transcriptional regulator CRE1, known to act as a repressor in the process of carbon catabolite repression. The parental PC9 and the two transformants were grown on microcrystalline cellulose and wheat straw (3 replicates each) and the content of the resulted secretomes was analyzed by LC-MS/MS. An extensive range of carbohydrate active enzymes (CAZymes) were affected by the modification of cre1 expression levels. The three fungi revealed also differentiation towards the carbon source used in the growth media.

2018-08-06 | PXD010100 | Pride

Transcriptomes of Pleurotus ostreatus in four different development stages (mycelium, primordium, young fruit body, mature fruit body)

Project description:To elucidate the mechanisms of fruit body development in Pleurotus ostreatus, the transcriptomes of four different development stages including mycelium, primordium, young fruit body, and mature fruit body were obtained by RNA-seq.

2018-04-12 | GSE113026 | GEO

Gene expression induced by blue light stimulation to oyster mushroom mycelia

Project description:Photoresponse mechanism of oyster muchroom mycelia was studied using a custom microarray prepared on the basis of the genome information (Pleurotus ostreatus PC15 v2.0) in JGI Genome Portal. Blue light stimulation to the mycelia caused the up-regulation and down-regulation of genes expression. Particulary, the genes coding rate-controlling enzymes in glycolysis, pentose phosphate, and sikimic acid pathways were up-regulated to accumulate shikimic acid dramatically.

2017-02-09 | GSE58522 | GEO

Impact of transposable elements in Pleurotus ostreatus transcriptome profiles

2016-05-19 | GSE81586 | GEO

Proteomic analysis of Pleurotus ostreatus grown on glucose and xy-lose mixtures in submerged fermentation

Project description:Pleurotus ostreatus, an edible white-rot fungus of great commercial and nutritional value, grows by metabolizing mainly glucose and xylose, the two major sugars in lignocellulosic biomass. In this study, a comparative proteomic analysis of P. ostreatus grown in submerged fermentation on medium with glucose, xylose and mixtures of them as carbon sources was conducted. In the same conditions, the metabolic response of the fungus was evaluated in the production of the main nutritional components of the fungus such as proteins, lipids, intracellular and extracellular polysaccharides. Xylose is found to up-regulate the tricarboxylic acid cycle whereas glucose upregulates the glycolysis cycle. An analysis of the upregulated proteins through the STRING database revealed that xylose upregulates mostly proteins related to amino acid biosynthesis. Leucine, Valine and Isoleucine biosynthesis pathways were found to be the most triggered pathway. These findings provide new insights into the proteomic and metabolic response of the fungus to the major sugars of lignocellulosic biomass. Based on these results, the metabolism of P. ostreatus could be turned towards a specific biological process using a suitable substrate. In addition, from proteomic analysis, strategies for genetic engineering of the strain could be adopted aiming to enhance protein expression, secretion and biotechnological-related pathways of the strain, a very constructive tool for the food industry.

2024-11-19 | PXD049213 | Pride

RNA-seq profiling of Pleurotus ostreatus exposed to BBP with or without cytochrome P450 inhibition

Project description:This study provides transcriptomic insights into the degradation of butyl benzyl phthalate (BBP) by the white-rot fungus Pleurotus ostreatus. RNA-seq was conducted for samples treated with BBP alone or in combination with the cytochrome P450 inhibitor piperonyl butoxide (PB). The aim was to investigate the role of fungal enzymes in BBP metabolism. Differentially expressed genes related to xenobiotic degradation, oxidative stress, and P450 activity were identified and analyzed.

2025-08-01 | GSE302615 | GEO

Transposon-associated epigenetic silencing during Pleurotus ostreatus life cycle

Project description:In this study, we analyzed the genome-wide epigenetic and transcriptional patterns of the white-rot basidiomycete Pleurotus ostreatus throughout its life cycle. Our results performed by using high-throughput sequencing analyses revealed that strain-specific DNA methylation profiles are primarily involved in the repression of transposon activity, and suggest that 21 nt small RNAs play a key role in transposon silencing.Furthermore, we provide evidence that transposon-associated DNA methylation, but not sRNA production, is directly involved in the silencing of genes surrounded by transposons. Finally, we identified key genes activated in the fruiting process through the comparative analysis of transcriptomes.

2018-05-15 | GSE112588 | GEO

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