Browse
Submit Data
Databases
API
Help

Dataset Information

0 Views

0 Connections

0 Citations

0 Reanalyses

0 Downloads

Omics score: 0

Caenorhabditis elegans

ABSTRACT: RNA-seq of C. elegans worms treated with RNAi toward daf-2 or cav-1 or left untreated (EV) to compare gene expression profiles

PROVIDER: PRJNA390548 | ENA |

REPOSITORIES: ENA

ACCESS DATA

Json Xml

Dataset's files

Source:

			Action	DRS
	SRR5684033.fastq.gz	Fastqsanger.gz
	SRR5684034.fastq.gz	Fastqsanger.gz
	SRR5684035.fastq.gz	Fastqsanger.gz
	SRR5684036.fastq.gz	Fastqsanger.gz
	SRR5684037.fastq.gz	Fastqsanger.gz

Items per page:

1 - 5 of 6

Similar Datasets

RNA-seq of C. elegans worms treated with RNAi toward daf-2 or cav-1 or left untreated (EV) to compare gene expression profiles

Project description:We observed functional outcomes of cav-1 knockdown and wanted to characterize changes in gene expression profiles following this manipulation. To address this, we treated nematodes with daf-2 or cav-1 RNAi and compared gene expression profiles to these of untreated worms. The knockdown of cav-1 resulted in modulation in the expression of 92 genes, and some of them overlapped with genes which were affected by knockdown of daf-2.

2017-06-19 | GSE100045 | GEO

mRNA-seq analysis of early adult C. elegans following extended L1 arrest with reduced insulin/insulin-like signaling

Project description:We characterized the effects of early-life starvation and reduced insulin/insulin-like signaling (IIS) during larval development on adult gene expression using mRNA-seq of whole worms. In our two-factor design, 'starved' worms were cultured without food (E. coli) in L1 arrest for eight days, and 'control' worms were starved overnight for synchronization. Both populations of worms were fed ad libitum with either empty vector (EV; negative control) or daf-2/InsR RNAi food (reduced IIS). RNAi was used rather than a daf-2 mutant so that the results would not be confounded by daf-2 function during L1 arrest, instead disrupting daf-2 only after starvation in fed, developing larvae. Upon reaching adulthood, animals were collected for transcript profiling.

2022-11-08 | GSE178170 | GEO

RNAi knock down of daf-2, daf-3, daf-16 in C. elegans over 0-48 hours

Project description:Comparison of sterile worms grown on Control Vector, daf-2 RNAi, and daf-2 + daf-16 RNAi. Synchronized fer-15(b26); fem-1(hc17) animals were grown on RNAi bacteria at 25ºC, induced with IPTG on Day 1 of adulthood, and collected from 0-48 hours (10 time points) and 0-196 hours (10 time points); RNAi bacteria was supplemented as necessary for later time points. Worms were floated off lawns with M9 buffer, centrifuged, and washed again in M9. The pelleted worms were dissolved in Trizol (Gibco) and frozen in liquid nitrogen.Time courses were compared with mixed references (collected from 1/2 of each of the time course samples)

2003-07-17 | E-MEXP-8 | biostudies-arrayexpress

RNAi knock down of daf-16 in C. elegans over 0-192 hours

2003-07-17 | E-MEXP-9 | biostudies-arrayexpress

Caenorhabditis elegans

Project description:Adult C. elegans: Control daf-2 mutants treated with daf-16 RNAi vs. daf-2 mutants treated with empty vector RNAi

| PRJNA123787 | ENA

Histone Demethylase UTX-1 Regulates the Caenorhabditis elegans Lifespan by Targeting the Insulin/IGF-1 Signaling Pathway

Project description:Epigenetic modifications are thought to be important for gene expression changes during development and aging. However, besides the Sir2 histone deacetylase in somatic tissues and H3K4 trimethylation in germlines, there is scant evidence implicating epigenetic regulations in aging. The insulin/IGF-1 signaling (IIS) pathway is a major lifespan regulatory pathway. Here we show that progressive increases in gene expression and loss of H3K27me3 on IIS components are due, at least in part, to increased activity of the H3K27 demethylase UTX-1 during aging. RNAi of the utx-1 gene extended the mean lifespan of C. elegans by ~30%, dependent on DAF-16 activity and not additive in daf-2 mutants. The loss of utx-1 increased H3K27me3 on the Igf1r/daf-2 gene and decreased IIS activity leading to a more "naive" epigenetic state. Like stem cell reprogramming, our results suggest that reestablishing epigenetic marks lost during aging might help "reset" the developmental age of animal cells. Examination of H3K27me3 in young and old worms without or with Utx-1 RNAi.

2011-11-22 | E-GEOD-29896 | biostudies-arrayexpress

Methylation profiling of C.elegans to identify DAF-16 binding

Project description:Profiling of DAF-16 binding by comparison of DNA methylation of a C. elegans control strain expressing the DNA adenine methyltransferase (DAM) and an experimental strain expressing a DAF-16::DAM. Both strains were fed daf-2 RNAi to identify DAF-16 binding associated with long lived worms.

2010-03-01 | E-MEXP-2414 | biostudies-arrayexpress

Caenorhabditis elegans

Project description:C. elegans daf-10(m79) mutants vs wild-type worms

| PRJNA178552 | ENA

RNA-seq using the Cel-Seq2 method, of wild type and 35-polyglutamine (polyQ35) expressing C. elegans worms treated with RNAi toward anc-1, or left untreated (EV) gene expression profiles.

Project description:RNA-seq using the Cel-Seq2 method, of wild type and 35-polyglutamine (polyQ35) expressing C. elegans worms treated with RNAi toward anc-1, or left untreated (EV) gene expression profiles.

| PRJNA522451 | ENA

mRNA-seq analysis of Caenorhabditis elegans wild type N2, daf-16(mu86), daf-18(ok480) and daf-16(mu86); daf-18(ok480) starved L1 larvae

Project description:Embryos of wild type N2, daf-16(mu86), daf-18(ok480) and daf-16(mu86); daf-18(ok480) were collected by hypochlorite-treat gravid adult worms and were put in virgin S-basal with 0.1% EtOH at 20 °C. After hatching, those embryos entered L1 arrest. Arrested L1s were collected 16 hr post hypochlorite treatment (about 4 hr of starvation).

2025-04-05 | GSE281157 | GEO

OmicsDI is part of the ELIXIR infrastructure

OmicsDI is an Elixir interoperability service. Learn more ›

Tweets

OmicsDI Databases

PRIDE
PeptideAtlas
MassIVE
JPOST Repository
Physiome Model Repository

EGA
EVA
ENA
LINCS
PAXDB
Cell Collective

MetaboLights
Metabolomics Workbench
MetabolomeExpress
GNPS
BioModels
FAIRDOMHub

ArrayExpress
dbGaP
ExpressionAtlas
GEO
NODE

Information

Databases
Help
API
Contact us
Code on GitHub
Terms of use
Submit Data