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Sus scrofa

ABSTRACT: Effect of the addition of corn DDGS and different sources of fat to the feedstuff of pigs on backfat transcriptome.

PROVIDER: PRJNA394207 | ENA |

REPOSITORIES: ENA

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			Action	DRS
	SRR5826460.fastq.gz	Fastqsanger.gz
	SRR5826470.fastq.gz	Fastqsanger.gz
	SRR5826461.fastq.gz	Fastqsanger.gz
	SRR5826471.fastq.gz	Fastqsanger.gz
	SRR5826462.fastq.gz	Fastqsanger.gz

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Effect of the addition of corn DDGS and different sources of fat to the feedstuff of pigs on backfat transcriptome.

Project description:We compared the transcriptomes of the backfat from pigs obtaining different diet. Animals were divided into four feeding groups The diet differed among groups in terms of the presence of corn DDGS as well as the type of fat used. We observed upregulation of several genes engaged in metabolism of lipids, fatty acyl-CoA biosynthesis and organization of lipid particles in the samples from animals which did not obtain cDDGS in the feedstuff.

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Project description:Large White and Meishan pigs were either non-treated or injected with mammalian 1-24 ACTH (Immediate Synachten, Novartis France) at the dose of 250 µg per animal. Pigs were sacrificed either immediately after capture from their home cage (non-treated animals) or 1 hour following ACTH injection. Adrenal glands were immediately collected from pigs and frozen on dry ice and then stored at -80°C until RNA isolation. Keywords: stress response, adrenal, gene expression, pig

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Expression data from Sus scrofa atria

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DH06 Adrenal ACTH Sus scrofa

Project description:Large White and Meishan pigs were either non-treated or injected with mammalian 1-24 ACTH (Immediate Synachten, Novartis France) at the dose of 250 Âµg per animal. Pigs were sacrificed either immediately after capture from their home cage (non-treated animals) or 1 hour following ACTH injection. Adrenal glands were immediately collected from pigs and frozen on dry ice and then stored at -80Â°C until RNA isolation. Keywords: stress response, adrenal, gene expression, pig 47 samples

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Project description:Intramuscular fat variability in pigs

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Identification of candidate genes that specifically regulate subcutaneous and intramuscular fat deposition from transcriptomic and proteomic profiles in Dingyuan pigs

Project description:Purpose: This study conducted RNA-Seq analysis on Dingyuan pig individuals with different intramuscular fat content and backfat thickness, in order to find genes that specifically regulate subcutaneous fat and intramuscular fat deposition in pigs

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Differences in gene expression profiles for subcutaneous adipose, liver, and skeletal muscle tissues between Meishan and Landrace pigs with different backfat thicknesses

Project description:Backfat thickness is one of the most important traits of commercially raised pigs. Meishan pigs are renowned for having thicker backfat than Landrace pigs. To examine the genetic factors responsible for the differences, we first produced female crossbred pig lines by mating Landrace (L) × Large White (W) × Duroc (D) females (LWD) with Landrace (L) or Meishan (M) boars (i.e., LWD × L = LWDL for Landrace offspring and LWD × M = LWDM of the Meishan offspring). We confirmed that LWDM pigs indeed had a thicker backfat than LWDL pigs. Next, we performed gene expression microarray analysis (in both genetic lines) to examine differentially expressed genes (DEGs) in energy metabolism-related tissues, subcutaneous adipose (fat), liver, and longissimus dorsi muscle tissues. We analyzed the annotation of DEGs (2-fold cutoff) to functionally categorize them by Gene Ontology and Kyoto Encyclopedia of Genes and Genomes pathways. The number of DEGs in muscle tissues of both lines was much less than that in fat and liver tissues, indicating that DEGs was much lesser in muscle tissues (in both genetic lines) than in fat and liver tissues, thus indicating that DEGs in muscle tissues may not contribute much to differences in backfat thickness. In contrast, several genes related to muscle (in fat tissue) and fatty acid and glucose metabolism (in the liver) were more upregulated in LWDM pigs than LWDL pigs, indicating that those DEGs might be responsible for differences in backfat thickness. The different genome-wide, gene expression profiles in the fat, liver, and muscle tissues between breeds can provide useful information for pig breeders.

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