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Genomic analysis for hematopoietic stem and progenitors cells (HSPC) generated in vitro according to ex vivo expansion protocols and their comparison with HSPC obtained fresh

ABSTRACT: Genomic analysis for hematopoietic stem and progenitors cells (HSPC) generated in vitro according to ex vivo expansion protocols and their comparison with HSPC obtained fresh

PROVIDER: PRJNA420375 | ENA |

REPOSITORIES: ENA

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Genomic analysis for hematopoietic stem and progenitors cells (HSPC) generated in vitro according to ex vivo expansion protocols and their comparison with HSPC obtained fresh

Project description:Expansion for hematopoietic cells from umbilical cord blood is a strategy for use this cell source in clinic transplants, however, it is important to know about the genomic changes that can occur in expanded cells. In order to detect global expression profiles changes in hematopoietic stem and progenitors cells generated in vitro, we analyzed hematopoietics populations obtained by FACS in fresh from umbilical cord blood. HSC (fHSC) was defined as CD34+ CD38- CD71- CD45RA- Lin- and were cocultured with stromal cell line OP-9 plus FL, SCF, IL3, IL6, TPO, GMCSF and G-CSF by 7 days, after time we repurified HSC population by FACS using same immunophenotype (ivHSC). In other hand, fresh erythroid progenitors cells (fEPC) were identified as CD34+CD38+CD71+CD45RA- Lin- and fresh myeloid progenitors cells (fMPC) were identified as CD34+CD38+CD71-CD45RA+Lin-. In vitro progenitors cells (ivEPC and ivMPC) were obtained by culturing fHSC in Stemspan serum-free media plus SCF, TPO, IL6, FL and IL3 by 10 days, after time cells were repurified by FACS using same immunophenotype for fresh progenitors. In vitro generated cells were compared with their corresponding fresh population cells.

2018-06-05 | GSE107497 | GEO

mRNAs microarray from 5 fresh lung cancer tissues and 5 fresh non-tumor tissues.

Project description:We performed mRNAss microarray to analyze the differential mRNAs expression profiles according to the recommended protocols.

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Rawdata of mRNAs microarray from 3 fresh lung cancer tissues and 3 fresh non-tumor tissues.

Project description:We performed mRNAss microarray to analyze the differential mRNAs expression profiles according to the recommended protocols.

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MiRNA microarray analysis of 5 fresh lung cancer tissues and 5 fresh non-tumor lung tissues

Project description:We performed miRNAs microarray to analyze the differential miRNAs expression profiles according to the recommended protocols.

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Low-cost, low-input RNA-seq protocols perform nearly as well as high-input protocols

Project description:We sequenced mRNA according to several library prep protocols with known mixtures of two species of Drosophila in order to establish linear response in each protocol.

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Rawdata of miRNAs microarray from 3 fresh lung cancer tissues and 3 fresh non-tumor lung tissues

Project description:We performed miRNAs microarray to analyze the differential miRNAs expression profiles according to the recommended protocols.

2023-09-05 | GSE229302 | GEO

HSPC scRNA seq

Project description:HSPC scRNA seq

| PRJNA627730 | ENA

HSPC and CRPC

Project description:Genetic alteration between HSPC and CRPC

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ATAC-seq from erythroid cells generated through ex vivo differentiation

Project description:CD34+ Haematopoietic stem cells were differentiated under two ex vivo protocols to generate ATAC-seq data for machine learning of rules underlying open chromatin dynamics.

2020-07-08 | GSE136976 | GEO

Pericyte-like cells generated from human pluripotent stem cells support hematopoietic stem and progenitors ex vivo

Project description:Various mesenchymal cell types have been identified as critical components of the hematopoietic stem/progenitor cell (HSPC) niche. Although several groups have described the generation of mesenchyme from human pluripotent stem cells (hPSC), the capacity of such cells to support hematopoiesis has not been reported. Here we have demonstrated that distinct mesenchymal subpopulations co-emerge from mesoderm during hPSC differentiation. Despite co-expression of common mesenchymal markers (CD73, CD105, CD90, PDGFRβ), a subset of cells defined as CD146++CD140alow supported functional HSPC ex vivo while CD146-CD140a+ cells drove differentiation. The CD146++ subset expressed genes associated with the HSPC niche and high levels of the Wnt inhibitors. HSPC support was contact-dependent and was mediated in part through JAG1 expression. Molecular profiling revealed remarkable transcriptional similarity between hPSC-derived CD146++ and primary human CD146++ perivascular cells. The derivation of diverse pools of mesenchymal populations from hPSC opens potential avenues to model their developmental and functional differences and to improve cell-based therapeutics from hPSC.

2018-03-07 | GSE83443 | GEO

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