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Oryza sativa Argonaute 18 is a regulator of male sterility (sRNA-Seq)

ABSTRACT: Oryza sativa Argonaute 18 is a regulator of male sterility (sRNA-Seq)

PROVIDER: PRJNA421904 | ENA |

REPOSITORIES: ENA

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			Action	DRS
	SRR6365690.fastq.gz	Fastqsanger.gz
	SRR6365700.fastq.gz	Fastqsanger.gz
	SRR6365691.fastq.gz	Fastqsanger.gz
	SRR6365701.fastq.gz	Fastqsanger.gz
	SRR6365692.fastq.gz	Fastqsanger.gz

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Oryza sativa Argonaute 18 is a regulator of male sterility (RNA-Seq)

Project description:Oryza sativa Argonaute 18 is a regulator of male sterility (RNA-Seq)

| PRJNA421905 | ENA

Oryza sativa Argonaute 18 is a regulator of male sterility (sRNA-Seq)

Project description:Artificial miRNA mediated knock-down ago18 transgenic lines [ago18(1), ago18(2)] showed developmentally compromised phenotype in both vegetative and reproductive tissues compared to wild-type control. We have performed small RNA seq to investigate underlying molecular mechanism and to reveal functional role of AGO18 in rice.

2018-01-15 | GSE107904 | GEO

Oryza sativa Argonaute 18 is a regulator of male sterility (RNA-Seq)

Project description:Artificial miRNA mediated knock-down ago18 transgenic lines [ago18(1), ago18(2)] showed developmentally compromised phenotype in both vegetative and reproductive tissues compared to wild-type control. We have performed RNA seq to investigate underlying molecular mechanism and to reveal functional role of AGO18 in rice.

2018-01-15 | GSE107903 | GEO

mRNA cleavage by 21-nt phasiRNAs dependent on AGO1d is important for temperature-sensitive male sterility in rice

Project description:Temperature-sensitive male sterility is one of the core components for hybrid rice breeding based on two-line system. We previously found that knockout of ARGONAUTE 1d (AGO1d) caused temperature-sensitive male sterility in rice by influencing phased small interfering RNA (phasiRNA) biogenesis and function. However, the specific phasiRNAs and their targets underlying the temperature-sensitive male sterility in ago1d mutant remain to be determined. Here, we demonstrate that the ago1d mutant displays normal female fertility but complete male sterility at low temperature. Through a multi-omics analysis of small RNA, degradome, and transcriptome, we found that 21-nt phasiRNAs account for the greatest proportion of the 21-nt small RNA (sRNA) species in rice anthers and are sensitive to low temperature and markedly down-regulated in ago1d mutant. Moreover, we found that 21-nt phasiRNAs are essential for the mRNA cleavage of a set of fertility- and cold tolerance-associated genes, such as EDT1, TDR, OsPCF5 and OsTCP21, directly or indirectly determined by AGO1d-mediated gene silencing. The loss-of-function of 21-nt phasiRNAs can result in up-regulation of their targets and causes varying degrees of defects in male fertility and grain setting. Our results highlight the essential functions of 21-nt phasiRNAs in temperature-sensitive male sterility in rice and suggest their promising application in two-line hybrid rice breeding in the future.

2024-01-01 | GSE232779 | GEO

Distinct Functions of Argonaute Slicer in siRNA Maturation and Heterochromatin Formation

Project description:Small-RNA (sRNA)-guided transcriptional gene silencing by Argonaute (Ago)-containing complexes is fundamental to genome integrity and epigenetic inheritance. The RNA cleavage (“Slicer”) activity of Argonaute has been implicated in both sRNA maturation and target RNA cleavage. Typically, Argonaute slices and releases the passenger strand of duplex sRNA to generate active silencing complexes, but it remains unclear whether slicing of target nascent RNAs, or other RNAi components, also contributes to downstream transcriptional silencing. Here, we develop a strategy for loading the fission yeast Ago1 with a single-stranded sRNA guide, which bypasses the requirement for slicer activity in generation of active silencing complexes. We show that slicer-defective Ago1 can mediate secondary sRNA generation, H3K9 methylation, and silencing similar to or better than wild-type and associates with chromatin more efficiently. The results define an ancient and minimal sRNA-mediated chromatin silencing mechanism, which resembles the germline-specific sRNA-dependent transcriptional silencing pathways in Drosophila and mammals. This SuperSeries is composed of the SubSeries listed below.

2016-12-19 | GSE81734 | GEO

Analysis of sex chromosomes effect on hybrid male sterility

Project description:Sex chromosomes and more particularely the X chromosomes are known to have a major effect on hybrid male sterility. In this experiment by making use of the reciprocal hybrids between D. simulans and D. sechellia, we are showing the effect of these chromosomes on gene expression in male hybrids Keywords: X chromosome, hybrid

2009-11-01 | GSE4677 | GEO

Germ granule association drives small RNA specificity for a nuclear Argonaute protein [sRNA-seq]

Project description:Germ granule association drives small RNA specificity for a nuclear Argonaute protein [sRNA-seq]

| PRJNA998763 | ENA

pepper stamen LC-MSMS - Proteomic analysis reveals strong mitochondrial involvement in cytoplasmic male sterility of pepper (Capsicum annuum L

Project description:We used a high-throughput proteomics method called label-free to compare protein abundance across a pepper CMS line and its isogenic maintainer line.This study explained the mechanisms of cytoplasmic male sterility and contribute to the improvement of pepper hybrid breeding.

2017-09-01 | PXD006104 | Pride

Oxidative burst causes loss of tapetal Ubisch body and male sterility in rice

Project description:We identified the nup1 mutant rice that exhibited specific male sterility due to the absence of Ubisch body formation and pollen grain production. We cloned the causal gene and demonstrated that NUP1, a class III peroxidase predominantly expressed in the anther wall, mediated ROS scavenging. The loss-of-function of NUP1 resulted in ROS burst in anthers, which disrupted the oxidation-reduction process and carbohydrate and lipid metabolisms, and thereby led to loss of tapetal Ubisch bodies and ultimately resulted in male sterility in rice. Our study expands the understanding of the molecular mechanisms by which ROS homeostasis regulates cell metabolism, tapetal Ubisch body formation and male fertility in plants.

2024-06-30 | GSE250072 | GEO

The N-terminal extension of Arabidopsis ARGONAUTE 1 is essential for microRNA activities [sRNA-seq]

Project description:The N-terminal extension of Arabidopsis ARGONAUTE 1 is essential for microRNA activities [sRNA-seq]

| PRJNA934562 | ENA