Project description:The resveratrol-producing rice (Oryza sativa L.) inbred line, Iksan 526 (I.526), developed by the expression of the groundnut (Arachis hypogaea) resveratrol synthase 3 (AhRS3) gene in the japonica rice cultivar Dongjin, accumulated both resveratrol and its glucoside, piceid, in leaves and seeds. Especially, ultra-performance liquid chromatography (UPLC) analysis revealed that the biosynthesis of piceid reached peak levels at 20 days after heading (DAH) seeds. To investigate endogenous piceid biosynthesis genes (UGTs), total RNA samples of 20 DAH seeds was used for RNA-seq.
Project description:To evaluate the roles of gene regulation in Oryza sativa leaf, dynamic profiles of transcriptome were investigated in Oryza sativa L. spp. indica with different treatments, the aerial tissues of one-month-old plants from four different areas (groups 1–4) were treated with 0, 40 mL of 25% azoxystrobin, 0.01 g of VdAL, or 40 mL of 25% azoxystrobin plus 0.01 g VdAL, respectively.
Project description:Rice anthers at anthesis stage from the wild type and gori mutant anther (Dongjin cultivar) We collected the sample from our field and immediately froze the samples with liquid nitrogen.
Project description:We created a triple loss-of-function/knockout mutant targeting three rice (Oryza sativa japonica) genes simultaneously via the Clustered Regularly Interspaced Short Palindromic Repeats(CRISPR)/Cas9 system. The three selected genes are as follows: OsPRK1 (LOC_Os08g40990), OsPRK2 (LOC_Os06g45240), and OsPRK3 ((LOC_Os02g07810). These three OsPRKs are strongly transcriptional expressed in the mature anthers of rice (stages 13) and bi-/tricelluler pollen. The triple mutant of these OsPRKs does not produce self-fertilized seeds due to defects in the hydration and germination of pollen grains (male-sterile). This data is about RNA-sequencing transcriptome data about the Oryza sativa Dongjin used as a control and triple mutant of OsPRKs (OsPRK1/2/3). We sampled mature anther for the analysis.
Project description:Os02g31890 encodes a dehydration-responsive transcription factor (named ´ARID´) from rice (Oryza sativa, cv. Dongjin). Expression profiling was performed 90 min after the start of dehydration stress in roots of Oryza sativa wild-type plants (cv. Dongjin) and a knock-out (i.e. arid) mutant.
Project description:Comparative transcriptome sequencing in leaf and root tissues of Control and Salt-treated Oryza sativa generated 52.2 and 17.29 million high-quality reads.
