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Pathogen Trojan horse delivers bioactive host protein ZmMAC1 to alter maize (Zea mays) anther cell behavior in the male sterile mutant Zmmac1-1.

ABSTRACT: Pathogen Trojan horse delivers bioactive host protein ZmMAC1 to alter maize (Zea mays) anther cell behavior in the male sterile mutant Zmmac1-1.

PROVIDER: PRJNA431598 | ENA |

REPOSITORIES: ENA

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			Action	DRS
	SRR6511850.fastq.gz	Fastqsanger.gz
	SRR6511851.fastq.gz	Fastqsanger.gz
	SRR6511852.fastq.gz	Fastqsanger.gz
	SRR6511853.fastq.gz	Fastqsanger.gz
	SRR6511854.fastq.gz	Fastqsanger.gz

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Pathogen Trojan horse delivers bioactive host protein ZmMAC1 to alter maize (Zea mays) anther cell behavior in the male sterile mutant Zmmac1-1.

Project description:To determine if TH delivery of ZmMAC1 protein results not only in cytological but also molecular cell differentiation in Zmmac1-1, gene expression was determined using RNA sequencing of the infected anthers.

2018-03-07 | GSE109644 | GEO

Proteomic Changes of Wheat Male Sterile Anther Induced by Temperature

Project description:Wheat, an important human food crop, is very sensitive to temperature changes. Thermosensitive sterile lines (TMS) is a natural material for exploring the effects of anther development and temperature changes on male fertility. In order to study the possible molecular mechanism of regulating protein activity during anther fertility alteration, proteomics analysis based on high resolution mass spectrometry were used to study the binuclear anther of wheat YS3038.

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Expression data from LncRNA TROJAN knockdown breast cancer cells

Project description:Background: Estrogen receptor-positive (ER+) breast cancers represent approximately two-thirds of all breast cancers and have a sustained risk of late disease recurrence. Cyclin-dependent kinase 4 and 6 (CDK4/6) inhibitors have shown significant efficacy in ER+ breast cancer. However, their effects are still limited by drug resistance. In this study, we aim to explore the role of long noncoding RNA TROJAN in ER+ breast cancer. Methods: The expression level of TROJAN in breast cancer tissue and cell lines was determined by quantitative real-time PCR. In vitro and in vivo assays as well as patient derived organoids were preformed to explore the phenotype of TROJAN in ER+ breast cancer. The TROJAN-NKRF-CDK2 axis were screened and validated by RNA pull-down, mass spectrometry, RNA immunoprecipitation, microarray, dual-luciferase reporter and chromatin immunoprecipitation assays. Results: Herein, we showed that TROJAN was highly expressed in ER+ breast cancer. TROJAN promoted cell proliferation and resistance to a CDK4/6 inhibitor and was associated with poor survival in ER+ breast cancer. TROJAN can bind to NKRF and inhibit its interaction with RELA, upregulating the expression of CDK2. The inhibition of TROJAN abolished the activity of CDK2, reversing the resistance to CDK4/6 inhibitor. A TROJAN antisense oligonucleotide sensitized breast cancer cells and organoids to the CDK4/6 inhibitor palbociclib both in vitro and in vivo. Conclusions: TROJAN promotes ER+ breast cancer proliferation and is a potential target for reversing CDK4/6 inhibitor resistance.

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PDE4B deficiency aids macrophage Trojan horses to transfer Cryptococcus neoformans into the brain

Project description:Cryptococcal meningitis (CM) is a fatal compliation. Macrophages work as Trojan horses transferring Cryptococcus neoformans (C. neoformans) into the brain. Mechanisms for C. neoformans Trojan horses are largely elusive. In this study, we performed scRNA-Seq on immune cells infiltrated into the brain in a murine model of CM. Bioinformatics analysis reveals that phosphodiesterase 4B (PDE4B) ranks top in regulating macrophage Trojan horses. Melanin, a virulence factor for Cn, decreased PDE4B in macrophages. PDE4B inhibitor promoted the Cn Trojan horse across the blood-brain-barrier (BBB) in vitro and in vivo. As similar, PDE4B knockout increased fungi burden in the brain, which is, at least partially, rescued by macrophages depletion. In contrast, PDE4B activation diminished C. neoformans brain infection. Mechanistically, PDE4B inhibition increased CXCR4 and CCR7 on C. neoformans macrophage Trojan horses, a process regulated by the cAMP/PKA signaling pathway. Dexamethasone, commonly used to treat Pneumocystis infections in AIDS patients, significantly decreased PDE4B expression in macrophages. Overall, this study reveals that PDE4B plays a crucial role in C. neoformans macrophage Trojan horses and serves as a potential therapeutic target for CM.

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miR167 limits anther growth to potentiate anther dehiscence

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Project description:The RNA-seq was used to identify differentially regulated miRNAs between a male sterile and wild type tomato during anther development.

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Comparative expression profiling of miRNA during anther development in genetic male sterile and wild type cotton [small RNA]

Project description:Genetic male sterility (GMS) in cotton (Gossypium hirsutum) plays an important role in the utilization of hybrid vigor. However, the molecular mechanism of the GMS is still unclear. While numerous studies have demonstrated that microRNAs (miRNA) regulate flower and anther development, whether different small RNA regulations exist in GMS and its wild type is unclear. To investigate the global expression and complexity of small RNAs during cotton anther development, three small RNA libraries were constructed from the anthers of three development stages each from fertile wild type (WT) and its GMS mutant cotton.

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miR167 limits anther growth to potentiate anther dehiscence

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E-GEOD-109644

Project description:Pathogen Trojan horse delivers bioactive host protein ZmMAC1 to alter maize (Zea mays) anther cell behaviour in the male sterile mutant Zmmac1-1.

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Lolium perenne cv. Trojan

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