Project description:p130Cas is a polyvalent adapter protein essential for cardiovascular development, and with a key role in cell movement. In order to identify the pathways by which p130Cas exerts its biological functions in endothelial cells we mapped the p130Cas interactome and its dynamic changes in response to VEGF using high-resolution mass spectrometry and reconstruction of protein interaction (PPI) networks with the aid of multiple PPI databases. The work presented here was based on a collaboration between University College London and University College Dublin. Dr Ian Evans (first author) and Prof. Ian Zachary (lab head), can be contacted at: Centre for Cardiovascular Biology and Medicine, Division of Medicine The Rayne Building, University College London, London WC1E 6JJ, United Kingdom. Contact details for University College Dublin collaborators can be found below.
Project description:p130Cas is a polyvalent adapter protein essential for cardiovascular development, and with a key role in cell movement. In order to identify the pathways by which p130Cas exerts its biological functions in endothelial cells we mapped the p130Cas interactome and its dynamic changes in response to VEGF using high-resolution mass spectrometry and reconstruction of protein interaction (PPI) networks with the aid of multiple PPI databases. The work presented here was based on a collaboration between University College London and University College Dublin. Dr Ian Evans (first author) and Prof. Ian Zachary (lab head), can be contacted at: Centre for Cardiovascular Biology and Medicine, Division of Medicine The Rayne Building, University College London, London WC1E 6JJ, United Kingdom. Contact details for University College Dublin collaborators can be found below.
Project description:Recurrent spontaneous abortion (RSA) is a severe pregnancy disorder with heterogeneous etiologies. Emerging evidence has suggested a functional role of epigenetic modification in RSA development. N7-methylguanosine (m7G) in mRNA is one of the frequently reported epigenetic modifications closely associated with various human diseases. However, the role of m7G modification in the development of RSA remains poorly understood. In this study, we perform multi-omics analyses of decidua from RSA patients and healthy controls (HC), and report for the first time that m7G modification was associated with RSA development. MeRIP-seq verifies that the m7G modification features, such as sequence motifs, distribution regions, and distribution densities, were significantly different between the two groups. Notably, genes with altered m7G modification were mostly enriched in embryonic and systemic morphogenesis and development. Moreover, we found an increased expression of the m7G methyltransferase METTL1 in the RSA decidua. RNA sequencing (RNA-seq) further revealed a vastly changed transcriptome in the RSA decidua. Conjoint analysis of MeRIP-seq and RNA-seq further showed that the methylation levels and expression levels of 48 genes changed simultaneously in the RSA decidua. Furthermore, we identified 7 RSA-related genes such as SOCS3, RASA1, FLT1, and JUNB were downregulated, while GNLY was up-regulated in RSA decidua. More importantly, METTL1 showed high-affinity binding to target mRNAs harboring altered m7G modification, indicating dysregulated RSA-genes may be driven by METTL1-mediated m7G modification. These findings provide novel insight into the functional role of m7G modification in RSA pathogenesis.
Project description:A genetically diverse strain (labelled as London) of the phytophagous mite Tetranychus urticae was transferred from its common host (bean) to other host plants (cotton, maize or soy). Three different host plant species were included in the experimental set-up: cotton (Gossypium spp.), maize (Zea mays cv. Ronaldinio) and soy (Glycine max cv. Merlin). Five generations after host-transfer, total RNA of all mite populations (London, London-SOY, London-MAIZE and London-COTTON) was collected and used in a genome-wide gene expression microarray (Sureprint G3 microarray, Agilent) Microarray analysis revealed large-scale differential expression of genes coding for enzymes of detoxification families, secreted proteins with unknown functions and regulatory enzymes.
Project description:250 adult T. urticae females from the London strain (grown on acyanogenic P. vulgaris cv. Prelude bean plants) were transferred to cyanogenic P. lunatus cv. 8078 bean plants. Thirty-five generations after the host transfer, total RNA was extracted from mites growing on both bean species (London and London-CYANO strain) and used in in a genome-wide gene expression microarray (Sureprint G3 microarray, Agilent) experiment to assess significantly differentially expressed genes (FC ≥ 2 and FDR-corrected p-value < 0.05) between mites grown on P. vulgaris (cv. Prelude) bean plants (London strain) and mites grown for 35 generations on P. lunatus (cv. 8078) bean plants (London-CYANO strain).
Project description:The present study aimed to investigate the profile of serum-circulating miRNAs and their target genes and elucidate their role in infected and non-infected C. trachomatis RSA patients by microarray miRNAs and mRNAs were found to be differentially expressed in C. trachomatis-positive RSA patients Non-heparinized blood samples were collected from 25 RSA patients with history of three or more consecutive abortions
Project description:Transcriptional profiling of Coxiella burnetii phase I (RSA 493) submitting either to Cold and Heat shock comparing to control untreated Coxiella burnetii phase I (RSA 493) grown at 35°C.