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Zea mays

ABSTRACT: Zea mays B73, seedling root tip (0-3mm), sorted nuclei, CENH3 ChIP

PROVIDER: PRJNA474205 | ENA |

REPOSITORIES: ENA

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Dataset's files

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			Action	DRS
	SRR7266940_1.fastq.gz	Fastqsanger.gz
	SRR7266940_2.fastq.gz	Fastqsanger.gz
	SRR7266941_1.fastq.gz	Fastqsanger.gz
	SRR7266941_2.fastq.gz	Fastqsanger.gz
	SRR7266942_1.fastq.gz	Fastqsanger.gz

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Project description:Conventional cellular transcriptome profiling (RNA-seq) data reports “steady-state” transcript abundance rather than actual transcription rates in the nucleus. It also lacks sensitivity to detect unstable and low-abundance RNA species. We generated these maize (Zea mays L.) B73 total cellular RNA samples, extracted directly from frozen 1-mm root tips, as a parallel experiment to compare to two other types of RNA extracted from isolated nuclei.

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Centromere location in Arabidopsis is unaltered by extreme divergence in CENH3 protein sequence

Project description:We report the binding profile of A. thaliana CENH3, L. oleraceum CENH3 and Z. mays CENH3 in the genetic background of A. thaliana Col ecotype. The L. oleraceum CENH3 and Z. mays CENH3 ChIP was performed in a cenh3 homozygous mutant background expressing CENH3 homologs as transgenes.

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Zea mays Transcriptome or Gene expression

Project description:In this study a transcriptomic approach (RNA-sequencing) was utilized to elucidate molecular responses of maize (Zea mays L.) primary roots of the inbred line B73 to water deficit to gain a better understanding of the mechanisms underlying drought tolerance. Kernels of the maize inbred line B73 were germinated in paper rolls soaked with distilled water until seedlings had a primary root length of 2 to 4 cm. For mild and severe water deficit conditions, seedlings were transferred to PEG8000 solution with water potentials of -0.2 MPa and -0.8 MPa, respectively. Water deficit treatment was applied for 6 h and 24 h. Each treatment was performed in four biological replicates each consisting of 10 roots.

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