Dataset Information

Transcriptome of day 6 EBs [WT, hWDR5iRescue,Wdr5 KO clone #3 with or without early (T12h) and late rescue (T48h)]

ABSTRACT: Transcriptome of day 6 EBs [WT, hWDR5iRescue,Wdr5 KO clone #3 with or without early (T12h) and late rescue (T48h)]

PROVIDER: PRJNA477503 | ENA |

REPOSITORIES: ENA

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Dataset's files

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			Action	DRS
	SRR7411930.fastq.gz	Fastqsanger.gz
	SRR7411940.fastq.gz	Fastqsanger.gz
	SRR7411931.fastq.gz	Fastqsanger.gz
	SRR7411941.fastq.gz	Fastqsanger.gz
	SRR7411932.fastq.gz	Fastqsanger.gz

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Project description:The ability of cells to perceive and translate versatile cues into differential chromatin and transcriptional states is critical for many biological processes1-4. In plants, timely transition to a flowering state is crucial for successful reproduction5-7. EARLY BOLTING IN SHORT DAY (EBS) is a negative transcriptional regulator that prevents premature flowering in Arabidopsis8,9. Here, we revealed that bivalent bromo-adjacent homology (BAH)-plant homeodomain (PHD) reader modules of EBS bind H3K27me3 and H3K4me3, respectively. A subset of EBS-associated genes was co-enriched with H3K4me3, H3K27me3, and the Polycomb repressor complex 2 (PRC2). Interestingly, EBS adopts an auto-inhibition mode to mediate its binding preference switch between H3K27me3 and H3K4me3. This binding balance is critical because disruption of either EBS-H3K27me3 or EBS-H3K4me3 interaction induces EBS-mediated early floral transition. This study identifies a single bivalent chromatin reader capable of recognizing two antagonistic histone marks and reveals a distinct mechanism of interplay between active and repressive chromatin states.The ability of cells to perceive and translate versatile cues into differential chromatin and transcriptional states is critical for many biological processes1-4. In plants, timely transition to a flowering state is crucial for successful reproduction5-7. EARLY BOLTING IN SHORT DAY (EBS) is a negative transcriptional regulator that prevents premature flowering in Arabidopsis8,9. Here, we revealed that bivalent bromo-adjacent homology (BAH)-plant homeodomain (PHD) reader modules of EBS bind H3K27me3 and H3K4me3, respectively. A subset of EBS-associated genes was co-enriched with H3K4me3, H3K27me3, and the Polycomb repressor complex 2 (PRC2). Interestingly, EBS adopts an auto-inhibition mode to mediate its binding preference switch between H3K27me3 and H3K4me3. This binding balance is critical because disruption of either EBS-H3K27me3 or EBS-H3K4me3 interaction induces EBS-mediated early floral transition. This study identifies a single bivalent chromatin reader capable of recognizing two antagonistic histone marks and reveals a distinct mechanism of interplay between active and repressive chromatin states.v

Dataset Information

Transcriptome of day 6 EBs [WT, hWDR5iRescue,Wdr5 KO clone #3 with or without early (T12h) and late rescue (T48h)]

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