Project description:Key genes involved in symbiosis have been lost in nonmycorrhizal plants such as Arabidopsis thaliana. We studied the effects on gene expression in A. thaliana expressing an abbreviated, functional version of one of genes, the transcription factor Interacting Protein of DMI3 (IPD3-min), under low nutrient conditions in the presence and absence of a mycorrhizal fungi, Rhizophagus. We conducted the same transcriptome analysis with a cyclops-4 knockout mutant in Lotus japonicus to compare with the expression profile of a mycorrhizal host model lacking IPD3.
Project description:To identify the regulatory targets of the R2R3-Myb transcription factor, LjMyb14, the gene was constitutively over-expressed in Lotus japonicus under the Lotus ubiquitin promoter.
Project description:To identify the regulatory targets of the R2R3-Myb transcription factor, LjMyb14, the gene was constitutively over-expressed in Lotus japonicus under the Lotus ubiquitin promoter. The gene expression levels of three biological replicates of the Lotus japonicus (MG20) were averaged and compared to the the gene expression levels of three independent lines of Lotus japonicus japonicus constituitively over expressing LjMyb14 using the Lotus ubiquitin promoter.
Project description:Lotus japonicus is a model legume broadly used to study transcriptome regulation under different stress conditions and microorganism interaction. Understanding how this model plant respond gainst alkaline stress will certainly help to develop more tolerant cultivars in economically important Lotus species as well as in other legumes. In order to uncover the most important response mechanisms activated during alkaline stress, we explored by microarray analysis the transcriptome regulation occurring in the phenotypically contrasting ecotypes MG-20 and Gifu B-129 of L. japonicus after 21 days of alkaline stress.
Project description:Lotus japonicus is a model legume broadly used to study transcriptome regulation under different stress conditions and microorganism interaction. Understanding how this model plant protects itself against pathogens will certainly help to develop more tolerant cultivars in economically important Lotus species as well as in other legumes. In order to uncover the most important defense mechanisms activated upon bacterial attack, we explored by microarray analysis the transcriptome regulation occurring in the phenotypically contrasting ecotypes MG-20 and Gifu B-129 of L. japonicus after inoculation with the non-pathogenic strain Pseudomonas syringae DC3000 pv. tomato.
Project description:The combined effects of high temperature stress from global warming and phosphorus (P) deficiency due to limited P rock reserves pose a critical threat to modern agriculture. However, the understanding of the effect of the combined stress on plants are limited. Here, we performed hydroponic culture to investigate the physiological and transcriptional response of the model legume Lotus japonicus to simultaneous moderate high temperature and low P conditions. While both elevated temperature and low P individually reduced shoot biomass, their combination alleviated growth reduction, with greater shoot biomass observed under combined stress than under low P alone. Lotus japonicus enhanced root-secreted acid phosphatase activity in response to low P alone and increased organic acid exudation rate, such as malate and citrate, under elevated temperature, regardless of P supply. We explored root transcriptome, and detected an up-regulated PEPC1 gene, encoding phosphoenolpyruvate carboxylase which suggested to be related with organic acid root exudation. Additionally, elevated temperature also increased metal ion translocation and absorption, anthocyanin levels, and carbohydrate transport in shoots. Transcriptomic analysis revealed that low P stress altered heat shock protein (HSP) expression, likely due to reduced ATP availability. This study demonstrates that L. japonicus employs distinct and overlapping strategies to adapt to the combined stresses of elevated temperature and P deficiency, including adjustments in exudation patterns, nutrient transport, and stress-responsive gene expression.
Project description:The present dataset is composed of Lotus japonicus root exudate samples under different nitrogen states: starved (no nitrogen), inorganic N (KNO3), symbiotic N (inoculation with M. loti), and inorganic/symbiotic (KNO3 + M. loti). The samples were analyzed by ultra-high-performance liquid chromatography (UHPLC) coupled to a quadrupole time-of-flight mass spectrometer (qToF MS, Bruker Compact) with electrospray ionization.