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Umbilical cord as a long-term source of activatable MSC for immunomodulation

ABSTRACT: Umbilical cord as a long-term source of activatable MSC for immunomodulation

PROVIDER: PRJNA483542 | ENA |

REPOSITORIES: ENA

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Umbilical cord as a long-term source of activatable MSC for immunomodulation

Project description:To facilitate umbilical cord explant culture as a long-term source for mesenchymal stromal cells, we compared the transcriptome of mesenchymal stromal cell explant cultures (MSC-EM) initiated 14 days (early) post preparation, 2 months later (late) and with INF-g and TNF-a (25 ng/mL) activated samples

2019-07-31 | GSE117901 | GEO

Umbilical cord as a long-term source of activatable mesenchymal stromal cells for immunomodulation.

Project description: Not available

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RNA-sequencing of human umbilical cord-derived MSC (UCX) in normoxia and hypoxia

Project description:Human umbilical cord matrix-derived mesenchymal stromal cells (UCM-MSC) are advantageous since can be easily obtained and display special interest as universal and feasible add-on therapy for myocardial infarction (MI). In this study, UCM-MSC from two umbilical cords, UC-A and UC-B, were transplanted in a murine MI model to investigate consistency and durability of the therapeutic benefits. Both cellular products supported sustained and long-term beneficial therapeutic effect. In vitro, the two cell products displayed similar ability to induce the formation of vessel-like structures and comparable transcriptome in normoxia and hypoxia, apart from expression differences in a small subset of genes associated with MHC Class I. These findings support that UCM-MSC are strong candidates to assist the treatment of MI whilst calling for the discussion on methodologies to characterize and select best performing UCM-MSC before clinical application.

2021-01-07 | E-MTAB-9978 | biostudies-arrayexpress

Effect of MSC and MSC-derived extracellular vesicles on human blood coagulation

Project description:Mesenchymal stem cells (MSC) have emerged as potent therapeutic tool for a number of pathologies, including immune ones. However, unwelcome effects of MSC on the blood coagulation were revealed in some cases, which require more in-depth analysis. In this study, we explored the trombotic properties of human MSC from umbilical cord. We revealed strong procoagulant effects of umbilical cord MSC toward human and rat whole blood and platelets-free plasma using rotational thromboelastometry and thrombodynamics tests. The similar potentiation of clotting was demonstrated for MSC-derived extracellular vesicles (EV). In order to suggest approaches to avoid unwanted effects we studied the impact of heparin supplement on MSC/EV procoagulation properties. We found that therapeutic doses of unfractionated heparin injected in the patient's blood (administered in vivo) did not abrogate the procoagulant properties of MSC. Mass-spectrometry analysis of proteins of MSC and EV involved in coagulation-associated pathways was used to evaluate mechanisms of protrombotic effects.

2019-03-26 | PXD012768 | Pride

RNA-sequencing of porcine heart after myocardial infarction with or without human umbilical cord-derived MSC (hUCM-MSC) delivery

Project description:Human umbilical cord matrix-mesenchymal stromal cells (hUCM-MSC) have demonstrated beneficial effects in experimental acute myocardial infarction (AMI). In this study, we investigated the outcome of the delivery of hUCM-MSC after reperfusion in a translational model of AMI in swine. We have found that intracoronary injection of the cell product was associated with improved systolic function and that improvement in mechanical performance. This did not depend on the reduction of morphological infarct size alone, despite treated animals showing signs of less adverse remodeling.

2023-06-01 | E-MTAB-13022 | biostudies-arrayexpress

RNA-sequencing of porcine heart after myocardial infarction with or without human umbilical cord-derived MSC (hUCM-MSC) delivery

Project description:RNA-sequencing of porcine heart after myocardial infarction with or without human umbilical cord-derived MSC (hUCM-MSC) delivery

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RNA-sequencing of human umbilical cord-derived MSC (UCX) in normoxia and hypoxia

Project description:RNA-sequencing of human umbilical cord-derived MSC (UCX) in normoxia and hypoxia

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Proteomics of human adipose and umbilical cord mesenchymal stem cell-derived extracellular vesicles

Project description:MSC-EV exerts their effects by transferring cargo components into target cells. Given that extracellular vesicles (EV) can deliver diverse cargo to recipient cells and elicit similar therapeutic effects, we investigate the cargo of both adipose-derived MSC-derived EV (AMSC-EV) and umbilical cord-derived MSC-derived EV (HUMSC-EV).As a crucial component of EV cargo, proteins are known to exert significant influence on EV functions. We performed a protein analysis of MSC-EV.

2025-05-07 | PXD056371 | Pride

Exosomes from MSC facilitate diabetic wound healing by promoting angiogenesis

Project description:Mesenchymal stem cell (MSC)-derived exosomes had been reported to be a prospective candidate in accelerating diabetic wound healing. Hence, this study intended to explore whether exosomes originating from the human umbilical cord MSC (hucMSC) could display a superior proangiogenic effect on diabetic wound repair and its underlying molecular mechanism.

2022-03-04 | GSE197900 | GEO

Clinical investigation on nebulized human umbilical cord MSC-derived extracellular vesicles for pulmonary fibrosis treatment

Project description:Mesenchymal stromal cell-derived extracellular vesicles (MSC-EVs) are recognized as a promising strategy for cell-free therapy, however, their therapeutic role in pulmonary fibrosis remains unrevealed. Here, we report the safety and efficacy of MSC-EVs from human umbilical cord (hUCMSC-EVs) evaluated in mouse models and pulmonary fibrosis patients. We established a rigorous system to produce high-quality of hUCMSC-EVs, characterized by miRNA, protein, and metabolite profiles. When administered via nebulization, hUCMSC-EVs predominantly accumulated in murine lungs and ameliorated bleomycin-induced pulmonary fibrosis, with increased survival rate (from 20% to 80%), restored lung volume, and attenuated injury severity accompanied by elevated oxyhemoglobin saturation and improved pulmonary function evaluations. We performed a phase l clinical trial involving twenty-four patients in a randomized, single-blind, and placebo-controlled study to treat pulmonary fibrosis (MR-46-22-004531, ChiCTR2300075466). All participants tolerated the nebulized hUCMSC-EVs well, with no serious adverse events. Patients receiving the combined therapy of nebulized hUCMSC-EVs and routine treatment demonstrated significant improvements in both lung function indices (forced vital capacity and maximal voluntary ventilation) and respiratory health status (as measured by the Saint George's Respiratory Questionnaire and Leicester Cough Questionnaire. Overall, patients upon the additional therapy with nebulized hUCMSC-EVs gained significant benefits compared with those accepted only routine treatment. Remarkably, two patients with advanced post-inflammatory pulmonary fibrosis exhibited clinically significant regression on serial CT scans after hUCMSC-EV therapy. These findings suggest that nebulized hUCMSC-EVs could be used as a promising therapeutic strategy for treating pulmonary fibrosis diseases.

2025-05-07 | PXD063385 | Pride

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