Streptomyces sp. S10(2018)
Ontology highlight
ABSTRACT:
PROVIDER: PRJNA507310 | ENA |
REPOSITORIES: ENA
Dataset's files
| Action | DRS | |||
|---|---|---|---|---|
| Other | ||||
| SRR8255522.fastq.gz | Fastqsanger.gz |
Items per page: 5 1 - 2 of 2 |
Similar Datasets
Project description:This study is aimed to isolate marine actinomycetes from sediments from Andaman and the Gulf of Thailand. All 101 marine actinomycetes were screened for anti-biofilm activity. Streptomyces sp. GKU223 showed significantly inhibited biofilm formation of S. aureus. The evaluation of supernatants of anti-biofilm activity produced by Streptomyces sp. GKU223 has been performed. Since the interaction between marine actinomycetes and biofilm forming bacteria has never been investigated, proteomic analysis has been used to identify whole cell proteins involved in anti–biofilm activity. Understanding the interaction at molecular level will lead to sustainably use for anti-biofilm producing marine actinomycetes in pharmaceutical and medicinal applications in the future.
2018-08-20 | PXD010815 | JPOST Repository
Project description:This study is aimed to isolate marine actinomycetes from sediments from Andaman and the Gulf of Thailand. All 101 marine actinomycetes were screened for anti-biofilm activity. Streptomyces sp. GKU 257-1 showed significantly inhibited biofilm formation of E. coli. The evaluation of supernatants of anti-biofilm activity produced by Streptomyces sp. GKU 257-1 has been performed. Since the interaction between marine actinomycetes and biofilm forming bacteria has never been investigated, proteomic analysis has been used to identify whole cell proteins involved in anti–biofilm activity. Understanding the interaction at molecular level will lead to sustainably use for anti-biofilm producing marine actinomycetes in pharmaceutical and medicinal applications in the future.
2019-09-07 | PXD011027 | JPOST Repository
Project description:Activation of cryptic biosynthetic gene clusters (BGCs) in actinomycetes often requires strong and reliable promoters, yet native promoters from rare actinomycetes remain scarce. Here, we used transcriptome analysis of Actinoplanes siamensis TBRC 4237 to identify highly expressed genes and selected 22 upstream regions as candidate constitutive promoters. Their activities were evaluated in Streptomyces coelicolor M1146 using indigoidine synthetase and latex-clearing protein (LCP) as heterologous reporters. Thirteen promoters drove indigoidine production at levels exceeding those of kasOp* and ermEp*, the widely used strong constitutive promoters in Streptomyces. Several promoters also yielded robust LCP activity, with promoter 650A achieving the highest expression across both reporter systems. Motif analysis showed no clear correlation between predicted -10/-35 elements and promoter strength, indicating additional regulatory features. These natural promoters provide valuable genetic parts for heterologous expression, BGC activation, and pathway refactoring in actinomycetes.
2026-02-25 | GSE320076 | GEO
Project description:Identifying cellular mechanisms maintaining HIV-1 latency in the viral reservoir is crucial for devising effective cure strategies. Here we developed a flow cytometry-fluorescent in situ hybridization (flow-FISH) approach using a combination of probes that detects abortive and elongated HIV-1 transcripts for ex vivo isolation and characterization of viral reservoir cells in peripheral blood from people with HIV-1. Following the isolation of three distinct cell populations from CD4+ T cells (i.e. cells harboring transcriptionally latent HIV-1, cells harboring transcriptionally active HIV-1, or uninfected cells), we determined their transcriptomic profile by RNA sequencing (RNAseq). Supervised gene expression analysis identified several differentially expressed mitochondrial genes in infected cell populations compared to uninfected cells, but also in latently infected compared to productively infected CD4+ T cells. Our transcriptomic profiling data shows an association between diminished mitochondrial functioning and the transcriptional activity of the viral reservoir. These findings underline the relevance of metabolic regulation in HIV-1 infection, and support the development of strategies modulating immunometabolism to target viral latency.
2024-12-17 | GSE260588 | GEO
Project description:Lysine acetylation is a dynamic, reversible post-translational modification that is known to play an important role in regulating the activity of many key enzymes in bacteria. Acetylproteome studies have been performed on some bacteria. However, until now there have been no data on the Actinomycetes, which are the major producers of therapeutic antibiotics. In this study, we investigated the first acetylproteome of the erythromycin-producing Actinomycete Saccharopolyspora erythraea using a high-resolution mass spectrometry-based proteomics approach. Using immune-affinity isolation of acetyl-peptides with an anti-acetyllysine antibody followed by nano-UPLC/MS/MS analysis, we identified 664 unique lysine acetylated sites on 363 proteins.
2025-11-27 | PXD001238 | Pride
Project description:Actinomycetes sequencing
| PRJNA622313 | ENA