Project description:ATAC-seq was carried out to identify regions of nucleosome depletion – marking sites of enhancers and promoters – in the MACS populations of CD4, CD8, CD14, CD19 and Natural Killer (NK) immune cells
Project description:Low-input bulk ATAC-seq of EPCR+ and EPCR– subsets of HSC and MPP1-6 populations was performed to compared the chromatin accessibility differences between these cell types.
Project description:The goal of the experiment was to understand the epigenetic effects of PU.1 haploinsufficiency on pro-B cells. The RS4:11 cell line was edited both mono and biallelicaly via electroporation of Cas9 and guides. Following editing, aliquots of unedited (SPI1 +/+), mono (SPI1 +/-) and biallellicaly edited (SPI1 -/-) cells were lysed before undergoing the transposition reaction. After transposition, the ATAC-seq libraries were purified and then amplified via PCR. Libraries were sequenced using the Illumina Novaseq platform.
Project description:CD34+ hematopoietic stem and progenitor cells (HSPCs) were purified from two Carnegie stage 22 fetal liver donors and processed using the 10X Genomics single cell ATAC-seq platform.