Ontology highlight
ABSTRACT:
PROVIDER: PRJNA522700 | ENA |
REPOSITORIES: ENA
Dataset's files
| Action | DRS | |||
|---|---|---|---|---|
| SRR8590620.fastq.gz | Fastqsanger.gz | |||
| SRR8590630.fastq.gz | Fastqsanger.gz | |||
| SRR8590640.fastq.gz | Fastqsanger.gz | |||
| SRR8590621.fastq.gz | Fastqsanger.gz | |||
| SRR8590631.fastq.gz | Fastqsanger.gz |
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Project description:Cytosine base modifications 5-methylcytosine (5mC), 5-hydroxymethylcytosine (5hmC) and 5-formylcytosine (5fC) are present in mammalian DNA. Here, reduced bisulfite sequencing is developed for quantitatively sequencing 5fC at single-base resolution. This method is then applied with oxidative bisulfite sequencing to gain a map of 5mC, 5hmC and 5fC in mouse embryonic stem cells. 12 samples, reduced representation bisulphite treatment: 4 replicates each for bisulphite (BS), oxidative BS (oxBS) and reduced BS (redBS) for the detection of 5mC, 5hmC and 5fC. Mouse (strain B6C) embryonic stem cells.
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