Project description:The nacre color of shells has an effect on the pearl color in Hyriopsis cumingii, and is an important indicator for its value. However, little exosome and micro (mi)RNA information are available on nacre color formation in mussels. In this study, exosomes of mantles were extracted from white and purple mussels. High-throughput Illumina sequencing was performed on the white and purple mussel mantle exosomes.Moreover, miR-223 negatively regulated hcApo, which plays important roles in the absorption and transport of β-carotene in H. cumingii. These results improve our understanding of the molecular mechanisms of nacre color formation in H. cumingii.
Project description:The proteome was based on the sequencing analysis of the mantle exosomes of purple and white Hyriopsis cumingii, and a total of 4861 proteins were obtained. After comparative analysis, 758 differential proteins were obtained (396 up-regulated proteins and 362 down-regulated proteins)
Project description:An integrated transcriptome analysis of immunological responses in pearl sac of pearl mussel Hyriopsis cumingii after mantle implantation
Project description:Mussel cell culture is a challenging problem because serums play a vital biological role in cell culture as autologous donors and immune agents. In this project, Label-free proteomic analysis was carried out on fetal bovine serum and hyriopsis cumingii serum, and significant different proteins and hyriopsis cumingii specific proteins were analyzed. Most differentially expressed proteins are involved in immune responses, cell differentiation, and calcium ion binding. In addition, HSP, CALR, APOB, C3 and other immune factors were significantly different.