Project description:We have employed a single-cell sequencing approach using 10X Genomics 5'scRNAseq to study expression of the Major Histocompatibility Complex (MHC) in peripheral blood mononuclear cells (PBMC) from Mauritian-origin cynomolgus and Indian-origin rhesus macaques. The MHC region has undergone a complex series of duplications in macaques relative to humans. Steady-state RNA levels of macaque MHC class I and class II transcripts vary over several orders of magnitude in whole blood and bulk PBMC. This study represents the first 5'scRNAseq analysis to quantify allele-specific levels of MHC transcripts in various PMBC subsets of macaques and provides insights into how they may shape immune responses.
2026-03-16 | GSE324697 | GEO
Project description:MHC Genometyping of Indian Rhesus Macaques Additional
Project description:We have employed a single-cell sequencing approach using 10X Genomics 5'scRNAseq to study expression of the Major Histocompatibility Complex (MHC) in peripheral blood mononuclear cells (PBMC) from Mauritian-origin cynomolgus and Indian-origin rhesus macaques. The MHC region has undergone a complex series of duplications in macaques relative to humans. Steady-state RNA levels of macaque MHC class I and class II transcripts vary over several orders of magnitude in whole blood and bulk PBMC. In this study, we compared transcript levels in untreated control PBMC with transcript levels after T cell activation, treatment with pro-inflammatory cytokines, or treatment with anti-inflammatory cytokines. Transcript levels for several minor MHC alleles were preferentially induced in monocytes and dendritic cells after treatment with pro-inflammatory cytokines or T cell activation.
Project description:Deep sequencing of mRNA from two macaques, crab-eating macaque and Indian rhesus macaque Analysis of ploy(A)+ RNA of different specimens:brain,ileum,kidney,liver,testes and white adipose for crab-eating macaque while brain,heart,,kidney,liver,quadriceps and testes for Indian rhesus macaque
Project description:Recent discoveries highlight the effectiveness of major histocompatibility complex (MHC)-E-restricted CD8+ T cell responses in controlling certain infections, particularly in a rhesus cytomegalovirus (RhCMV68-1)-vectored simian immunodeficiency virus (SIV) vaccine. In this context, these responses are preferentially elicited and are crucial for enabling vaccinated rhesus macaques to eradicate an SIV challenge. To harness human leukocyte antigen (HLA)-E-restricted CD8+ T cell responses for therapeutic purposes, it is essential to understand how these responses are primed, especially the mechanisms regulating MHC-E trafficking within endosomal pathways. Here, we identified a novel lysine/tryptophan-based motif in the HLA-E cytoplasmic tail that promotes rapid surface turnover via clathrin-mediated endocytosis. This motif, combined with strong binding peptides, also facilitates HLA-E recycling through a distinct valosin-containing protein-dependent pathway. Additionally, we show that this motif and its associated endosomal transport mechanisms are conserved in rhesus macaques and Mauritian-origin cynomolgus macaques. These findings advance our understanding of how MHC-E regulates immune functions through unconventional transport processes and offer insights for stimulating HLA-E-restricted CD8+ T cell responses in immunotherapy development.
Project description:A single AAV vector dose delivering broadly neutralizing antibody 3BNC117 at birth provided over 3 years of protection against HIV-like infection in infant rhesus macaques. Neonatal administration was most effective due to immune tolerance, which reduced anti-drug antibodies and enabled sustained bNAb expression. This approach offers a promising one-time gene therapy strategy to prevent perinatal and adolescent HIV-1 infections. A total of 65 rhesus macaques (Macaca mulatta) of Indian origin were utilized in this study
Project description:In this study three Indian rhesus macaques were infected with Simian Immunodeficiency Virus (SIVmac251). This generates a chronic infection mirroring AIDS in the animals. Prior to infection (day 0) and at two different time-points post-infection (day 21 and day 90), intestinal resection biopsies were performed. The intestinal tissue was then separated into different sub-sections including lamina-propria lymphocytes (LPL), intra-epithelial lymphocytes (IEL), stroma or matrix and epithelium. In this study we report the genome-wide transcriptional response at the two different time-points, specific to LPL, relative to pre-infection samples. For this purpose we have used the Affymetrix Rhesus Macaque GeneChip. 3 Rhesus Macaques prior to infection and 21 and 90 days of infection
