Project description:S.cerevisiae cells carrying a wild-type TOR1 allele or hyperactive TOR1L2134M allele were treated with the ER stressor, tunicamycin, and differential gene expression was examined between WT TOR1 and hyperactive TOR1LM We used microarrays to examine differential gene expression in cells expressing hyperactive TOR1LM following ER stress . We identified changes in expression of cell wall genes.
Project description:Sustained ER stress is tightly associated with hyperglycemia and type 2 diabetes.However, the molecular mechanisms remain largely unknown.In order to identify genes that are potentially involved in ER stress-associated hyperglycemia, transcriptomics analyses were performed using RNA-sequencing,which revealed that many genes were dys-regulated in the livers of mice treated with tunicamycin.
Project description:Label free proteomic analysis of C2C12 myoblasts treated with either Tunicamycin (0.2ug/ml) alone or with PERK AMG44 (2uM) for 8h and followed by 16h recovery.
Project description:This study treated the HCT116 cell line with Staurosporine (STS, a non-selective protein kinase inhibitor), Vorinostat (SAHA, an HDAC inhibitor), and Tunicamycin (Tuni, an N-glycosylation inhibitor) to investigate the phosphoproteome, N-glycoproteome, and acetylproteome.
Project description:Investigation of RNA-dependent proteins in the yeasts S.pombe and S.cerevisiae by using density gradient ultracentrifugation combined with mass spectrometry. The data consisted of three independent biological replicates of the corresponding wild-type strains. The analysis includes both a control group and a sample treated with RNases to identify RNA-dependent proteins.
Project description:NIH 3T3 cells were challenged with tunicamycin for 2, 5 or 10h. MICRO-RNAs that are upregulated or down regulated were identified by micro-ARRAY.