Browse
Submit Data
Databases
API
Help

Dataset Information

0 Views

0 Connections

0 Citations

0 Reanalyses

0 Downloads

Omics score: 0

RNA-seq of shRNA-mediated EXOSC10 and XRN2 knockdown

ABSTRACT: RNA-seq of shRNA-mediated EXOSC10 and XRN2 knockdown

PROVIDER: PRJNA541843 | ENA |

REPOSITORIES: ENA

ACCESS DATA

Json Xml

Dataset's files

Source:

			Action	DRS
	SRR9028500.fastq.gz	Fastqsanger.gz
	SRR9028501.fastq.gz	Fastqsanger.gz
	SRR9028502.fastq.gz	Fastqsanger.gz
	SRR9028503.fastq.gz	Fastqsanger.gz
	SRR9028504.fastq.gz	Fastqsanger.gz

Items per page:

1 - 5 of 6

Similar Datasets

RNA-seq of shRNA-mediated EXOSC10 and XRN2 knockdown

Project description:To identify changes in gene expression following EXOSC10 and XRN2 silencing, 3'-end RNA-sequencing was performed on RNA extracted from shEXOSC10 and shXRN2 cells.

2019-12-26 | GSE130865 | GEO

The immediate impact of exoribonucleolysis on nuclear RNA processing, turnover and transcriptional control revealed by rapid depletion of DIS3, EXOSC10 or XRN2 from human cells

Project description:Cell-based studies of human ribonucleases traditionally relies on methods that deplete proteins slowly. To assess their immediate roles in nuclear RNA biology, we engineered cells where the 3’->5’ exoribonucleases of the exosome complex, Dis3 and EXOSC10, can be eliminated within 60 minutes. Loss of Dis3 has the greatest impact, causing thousands of enhancer RNAs, intragenic transcripts, promoter upstream transcripts (PROMPTs) and products of premature cleavage and polyadenylation (PCPA) to accumulate. Interestingly, EXOSC10 only targets these substrates when Dis3 is absent, which is explained by its rapid mis-localization to the nucleoplasm following Dis3 loss. Direct detection of EXOSC10 substrates revealed a more specific role in trimming of short 3’ extensions on ribosomal and small nucleolar RNAs. Finally, the 5’-3’ exoribonuclease, Xrn2, has little activity on exosome substrates, but promotes termination following PCPA. Interestingly, Xrn2 is not rate limiting for PROMPT termination providing a distinction between sense and anti-sense transcriptional regulation.

2019-03-06 | GSE120574 | GEO

Total RNA-seq of shRNA-mediated TARBP2 knockdown

Project description:Total RNA-seq of shRNA-mediated TARBP2 knockdown

| PRJNA541848 | ENA

Nuclear RNA-seq of shRNA-mediated TARBP2 knockdown

Project description:Nuclear RNA-seq of shRNA-mediated TARBP2 knockdown

| PRJNA541853 | ENA

shRNA-mediated Myc knockdown and shRNA-mediated knockdown of Myc-induced lncRNA KTN1-AS1 in ST486 Burkitt lymphoma cells

Project description:shRNA-mediated Myc knockdown and shRNA-mediated knockdown of Myc-induced lncRNA KTN1-AS1 in ST486 Burkitt lymphoma cells

| PRJNA490714 | ENA

shRNA-mediated knockdown of PRMT5 gene in LNCaP cells

Project description:shRNA-mediated knockdown of PRMT5 gene in LNCaP cells

| PRJNA849221 | ENA

shRNA-mediated knockdown of MEP50 gene in LNCaP cells

Project description:shRNA-mediated knockdown of MEP50 gene in LNCaP cells

| PRJNA852249 | ENA

shRNA-mediated knockdown of MEP50 gene in LNCaP cells

Project description:We performed shRNA-mediated knockdown of MEP50 in LNCaP cells via dox-inducible expression system. We sought to compare transcriptome with- and without MEP50 knockdown across three biological replicates

2025-06-23 | GSE206820 | GEO

shRNA-mediated knockdown of PRMT5 gene in LNCaP cells

Project description:We performed shRNA-mediated knockdown of PRMT5 in LNCaP cells via dox-inducible expression system. We sought to compare transcriptome with- and without PRMT5 knockdown across three biological replicates

2025-06-13 | GSE206111 | GEO

XRN2

Project description:XRN2

| PRJNA769688 | ENA