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AP-1 Regulates Chromatin Accessibility to Promote Sarcomere Disassembly and Cardiomyocyte Protrusion during Zebrafish Heart Regeneration

ABSTRACT: AP-1 Regulates Chromatin Accessibility to Promote Sarcomere Disassembly and Cardiomyocyte Protrusion during Zebrafish Heart Regeneration

PROVIDER: PRJNA542058 | ENA |

REPOSITORIES: ENA

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			Action	DRS
	SRR9032270_1.fastq.gz	Fastqsanger.gz
	SRR9032270_2.fastq.gz	Fastqsanger.gz
	SRR9032280.fastq.gz	Fastqsanger.gz
	SRR9032280_1.fastq.gz	Fastqsanger.gz
	SRR9032280_2.fastq.gz	Fastqsanger.gz

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Project description:BACKGROUND: Mature mammalian cardiomyocytes (CMs) develop compact sarcomeric structures that inhibit proliferation. Consequently, CMs must dedifferentiate to a fetal-like state, which is accompanied by sarcomere disassembly, to enable successful cytokinesis. However, the regulation and coordination of CM dedifferentiation, cell cycle progression, and sarcomere reorganization remain unclear. METHODS: We generated adenovirus and adeno-associated virus (MyoAAV) vectors expressing YAP5SA and YAP5SA-S94A under Xon control for LMI070-inducible protein expression. We also developed MyoAAV-cTnT-Tuba1b-shRNA-miR30 for cardiomyocyte-specific knockdown (KD) of Tuba1b. These tools were used to investigated cardiomyocyte dedifferentiation, proliferation, and sarcomere disassembly. We also performed Cleavage Under Targets and Release Using Nuclease (CUT&RUN) to map the genome-wide binding sites of YAP5SA and YAP5SA-S94A, in combination with RNA sequencing to identify YAP target genes. In addition, time-course live-imaging analysis was used to evaluate microtubule and sarcomere dynamics in adult cardiomyocytes. RESULTS: We show that microtubule expression and network density decline with cardiac maturation. Overexpression of YAP5SA, a constitutively active YAP mutant, promotes microtubule growth by stabilizing the microtubule dynamics, leading to CM dedifferentiation, cell cycle re-entry and sarcomere disassembly. In contrast, colchicine blocks these processes and significantly attenuates YAP-induced cardiac regeneration. Live imaging reveals a distinct mode of sarcomere disassembly driven by enhanced microtubule polymerization, where microtubule plus-ends directly interact with α-actinin and displace α-actinin fragments, thereby facilitating sarcomere breakdown. Furthermore, the YAP-S94A mutation, which disrupts the YAP and TEAD interaction, significantly reduces YAP5SA-induced microtubule growth, sarcomere disassembly, and cell cycle activity. Mechanistically, CUT&RUN combined with RNA-seq identified direct YAP targets, including Ajuba and Tuba1b, which are critical for microtubule growth. CM-specific KD of Tuba1b attenuates YAP-driven sarcomere disassembly. CONCLUSIONS: These findings identify microtubule networks as an essential regulator modulating CM dedifferentiation and sarcomere reorganization, which is critical for CM cytokinesis and cardiac regenerative repair.

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AP-1 Regulates Chromatin Accessibility to Promote Sarcomere Disassembly and Cardiomyocyte Protrusion during Zebrafish Heart Regeneration

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