Browse
Submit Data
Databases
API
Help

Dataset Information

0 Views

0 Connections

0 Citations

0 Reanalyses

0 Downloads

Omics score: 0

ABSTRACT: Biosensor trigger library screening in mouse gut using engineered High-Throughput Memory System

PROVIDER: PRJNA542391 | ENA |

REPOSITORIES: ENA

ACCESS DATA

Json Xml

Dataset's files

Source:

			Action	DRS
	SRR9047380_1.fastq.gz	Fastqsanger.gz
	SRR9047380_2.fastq.gz	Fastqsanger.gz
	SRR9047390_1.fastq.gz	Fastqsanger.gz
	SRR9047390_2.fastq.gz	Fastqsanger.gz
	SRR9047400_1.fastq.gz	Fastqsanger.gz

Items per page:

1 - 5 of 172

Similar Datasets

Development and application of a Ligation-based Library vs Library high- throughput Yeast Two-Hybrid (LLL-Y2H) screening system

Project description:We developed a Ligation-based Library vs Library high-throughput Yeast Two-Hybrid (LLL-Y2H) screening system to explore protein interactions.

2019-12-30 | GSE93036 | GEO

E-GEOD-59846

Project description:Transcription profiling by high throughput of CD4 T memory cells before and after DNA methylation

2025-01-24 | E-GEOD-59846 | ExpressionAtlas

Interactome between ASFV and host immune pathway proteins

Project description:To systematically delineate the Protein–Protein Interaction (PPI) network between ASFV and host immune pathway proteins, and ASFV-ASFV PPI, we used the recombination-based library vs library high-throughput yeast two-hybrid (RLL-Y2H) screening system.

2023-10-23 | GSE220585 | GEO

E-PROT-144

Project description:Identification of carcinogenesis and tumor progression processes in pancreatic ductal adenocarcinoma using high-throughput proteomics

2024-06-05 | E-PROT-144 | ExpressionAtlas

Improved library preparation using the Nextera system

Project description:Improved Workflows for High Throughput Library Preparation using the Transposome-Based Nextera System

| PRJEB4315 | ENA

Genome wide transcriptome analysis of skin harboring tissue-resident memory CD8+ T cells

Project description:Comparison of the transcriptional profiles of full-thickness murine skin harboring tissue resident memory T cells exposed to specific or control trigger Expression profiling by high throughput sequencing

2014-09-11 | E-GEOD-60599 | biostudies-arrayexpress

Development and application of a Ligation-based Library vs Library high- throughput Yeast Two-Hybrid (LLL-Y2H) screening system

Project description:Development and application of a Ligation-based Library vs Library high- throughput Yeast Two-Hybrid (LLL-Y2H) screening system

| PRJNA359495 | ENA

Transcription profiling of human acute myeloid leukemia cells to identify compounds inducing the differentiation of acute myeloid leukemia cells

Project description:We developed a general approach to small molecule library screening called GE-HTS (Gene Expression-Based High Throughput Screening) in which a gene expression signature is used as a surrogate for cellular states and applied it to the identification of compounds inducing the differentiation of acute myeloid leukemia cells. In screening 1,739 compounds, we identified 8 that reliably induced the differentiation signature, and furthermore yielded functional evidence of bona fide differentiation. This SuperSeries is composed of the following subset Series:; GSE976: Gene Expression-Based High Throughput Screening: APL Treatment with Candidate Compounds; GSE982: Gene Expression-Based High Throughput Screening: HL-60 Cell Treatment with Candidate Compounds; GSE983: Gene Expression-Based High Throughput Screening: Primary Patient AML Blasts, Normal Neutrophils, and Normal Monocytes; GSE985: Gene Expression-Based High Throughput Screening: HL-60 Cells Treated with ATRA and PMA Experiment Overall Design: Refer to individual Series

2007-09-20 | E-GEOD-995 | biostudies-arrayexpress

Bacterial biosensor screening

Project description:Bacterial biosensor screening

| PRJEB66358 | ENA

Optimized RNA-targeting CRISPR/Cas13d technology outperforms shRNA in identifying functional circRNAs

Project description:Circular RNAs (circRNAs) are widely expressed, but their functions remain largely unknown. To study circRNAs in a high-throughput manner, short hairpin RNA (shRNA) screens1 have recently been used to deplete circRNAs by targeting their unique back-splicing junction (BSJ) sites. Here, we report frequent discrepancies between shRNA-mediated circRNA knockdown efficiency and the corresponding biological effect, raising pressing concerns about the robustness of shRNA screening for circRNA functional characterization. To address this issue, we leveraged the CRISPR/Cas13d system2 for functional study of circRNAs by optimizing the strategy for designing single guide RNAs to deplete circRNAs. We then performed shRNA and CRISPR/Cas13d parallel screenings and demonstrated that shRNA-mediated circRNA screening yielded a high rate of false positive phenotypes. Furthermore, using a CRISPR/Cas13d screening library targeting over 2,500 human hepatocellular carcinomas (HCC) related circRNAs, we identified a group of circRNAs, whose inhibition increased the therapeutic efficacy of sorafenib, an approved HCC drug. Collectively, these data demonstrate that CRISPR/Cas13d system is an effective approach to study the function of circRNAs in a high-throughput manner.

2020-12-06 | GSE162720 | GEO

OmicsDI is part of the ELIXIR infrastructure

OmicsDI is an Elixir interoperability service. Learn more ›

Tweets

OmicsDI Databases

PRIDE
PeptideAtlas
MassIVE
JPOST Repository
Physiome Model Repository

EGA
EVA
ENA
LINCS
PAXDB
Cell Collective

MetaboLights
Metabolomics Workbench
MetabolomeExpress
GNPS
BioModels
FAIRDOMHub

ArrayExpress
dbGaP
ExpressionAtlas
GEO
NODE

Information

Databases
Help
API
Contact us
Code on GitHub
Terms of use
Submit Data