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Ovis aries

ABSTRACT: Sheep Liver Transcriptome with High- and Low-RFI

PROVIDER: PRJNA547871 | ENA |

REPOSITORIES: ENA

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			Action	DRS
	SRR9291141_1.fastq.gz	Fastqsanger.gz
	SRR9291141_2.fastq.gz	Fastqsanger.gz
	SRR9301110_1.fastq.gz	Fastqsanger.gz
	SRR9301110_2.fastq.gz	Fastqsanger.gz
	SRR9302192_1.fastq.gz	Fastqsanger.gz

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MeDIP sequencing of Ovis aries

Project description:We have completed the high quality reference genome for domestic sheep (Oar v3.1). Early-stage Illumina GA sequence platform sequenced less reads in high GC content regions than in other regions. To read through higher GC content regions, we generated 2 Gb MeDIP-seq data for filling gaps in sheep reference genome assembly.

2014-06-06 | E-GEOD-56644 | biostudies-arrayexpress

MeDIP sequencing of Ovis aries

Project description:We have completed the high quality reference genome for domestic sheep (Oar v3.1).

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Transcriptome sequencing of Ovis aries

Project description:We have completed the high quality reference genome for domestic sheep (Oar v3.1) and performed a detailed survey of gene expression across different tissues. RNA-seq data of 7 tissue types from the reference female Texel and skin tissue from a Gansu alpine fine wool sheep were sequenced. Here is the part of the RNA-seq data sequenced in BGI, including 7 tissue types from the reference female Texel and skin type from a Gansu alpine fine wool sheep.

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Identification of reproduction-related genes in the hypothalamus of the sheep (Ovis aries) using the ONT full-length transcriptome sequencing technology

Project description:the hypothalamus tissues of high-reproduction small-tailed Han sheep and low-reproduction Wadi sheep were collected, and full-length transcriptome sequencing by Oxford Nanopore Technologies (ONT) was performed to explore the key functional genes associated with sheep fecundity. The differentially expressed genes (DEGs) were screened and enriched using DESeq2 software through Gene Ontology (GO) and Kyoto Encyclopedia of Genes and Genomes (KEGG).

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Aristaeus chip 1.0: an in situ synthesized oligonucleotide microarray for Ovis aries

Project description:Here we present a high-density in situ synthesized microarray for Ovis aries, named Aristaeus, designed by means of a pipeline of software instruments that, starting from non-annotated redundant EST sequences, selects oligonucleotides suitable for in situ generation on chip. The chip was tested by comparing the gene expression profiles of two sheep breeds with different phenotype, Sarda and Gentile di Puglia. We carried out microarray experiments on liver and udder tissues from lactating individuals and identified a relevant number of differentially expressed genes, all involved in metabolism pathways. The results are consistent with literature knowledge, while selected differential gene expressions have been confirmed by quantitative real-time polymerase chain reaction analyses. Tissue samples of liver were collected from 4 lactating individuals of two sheep (Ovis aries) breeds, Gentile di Puglia and Sarda. Biopsies of liver tissue were taken at second lactation stage (first record, stage 01: 6 days after lambing; second record, stage 02: 44 days after lambing) in both breeds. Tissues from liver were immersed in RNAlater (Sigma) immediately after biopsy and stored at -20°C. Samples were pooled by breed and then reverse labeled (cy5 and cy3), resulting in four raw data sets.

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