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Estrogen response in breast cancer cell line MCF-7 is dependent on NR2F2 [RNA-seq]

ABSTRACT: Estrogen response in breast cancer cell line MCF-7 is dependent on NR2F2 [RNA-seq]

PROVIDER: PRJNA548082 | ENA |

REPOSITORIES: ENA

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			Action	DRS
	SRR9276382_1.fastq.gz	Fastqsanger.gz
	SRR9276382_2.fastq.gz	Fastqsanger.gz
	SRR9276383_1.fastq.gz	Fastqsanger.gz
	SRR9276383_2.fastq.gz	Fastqsanger.gz
	SRR9276384_1.fastq.gz	Fastqsanger.gz

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Estrogen response in breast cancer cell line MCF-7 is dependent on NR2F2 [RNA-seq]

Project description:We show that most binding events of NR2F2 occur together with the ERα binding sites.To address the functional relationship between NR2F2 and ERα, we assessed the role of NR2F2 in oestrogen-induced growth in ER positive cell line MCF-7. The MTT experiment showed that inhibition of NR2F2 prevented the oestrogen-induced proliferation of MCF-7 cells.To further explore the effect of NR2F2 on estrogen response, We expanded our knockdown studies by performing RNA-seq analysis for MCF-7 cells transfected with control or NR2F2 shRNAs with or without E2.

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