Project description:We profiled accessibility by ATAC-seq of G1/S-synchronised HeLa cells that are wild-type (WT) or knock-out (KO) for the H3.3-specific chaperone HIRA and bear an exogenous H3.1-SNAP and H3.3-SNAP gene.
Project description:We over-expressed an epigenetic regulator in a glioblastoma (GBM) primary culture from an adult patient. These GBM cells have cancer stem cell phenotypes, as they have self-renewal properties and tumor initiation potential when transplanted in immunocompromised mice. ATAC-seq was performed on cells over-expressing the epigenetic regulator and control cells expressing EGFP. ATAC-Seq on glioblastoma cells that over-express EGFP or an epigenetic regulator.
Project description:We performed ATAC-seq assay on nuclei isolated from control and UTXKO mouse brown fat to delineate the role of UTX in setting chromatin accessibility. We analyzed the effects of UTX deficiency on a set of brown fat specific genes and observed reduced accessibility at ATAC-seq peaks and mRNA expression for many of these genes, including PRDM16 and PGC1a.
Project description:The serum of hibernating bears has been shown to exhibit cytoprotective, anticatabolic effects when applied to human or mouse skeletal muscle cells. It is also able to reduce mitochondrial respiration in cells obtained from bear adipose tissue. Its action notably might involve reprogramming of transcriptional profiles, as shown in brown bear adipocytes and more recently in human muscle cells. However, the effects of bear serum on human cells has not been evaluated yet at the proteome level. The main objective of this study was to fill this gap by comparing the effects of hibernating bear serum with those of non-hibernating bear serum and foetal bovine serum on human fibroblasts.
Project description:To uncover novel transcriptional regulatory mechanisms of brown adipogenesis, we performed Assay for Transposase-Accessible Chromatin using sequencing (ATAC-seq) to map genome-wide chromatin accessibility during the early stages of immortalized primary brown preadipocytes differentiation.Intriguingly, we identified a significant enrichment of the RUNX transcription factor binding motif within brown preadipocyte-specific peaks, suggesting a previously unrecognized role of the RUNX family as a potential barrier to brown adipogenesis
2026-02-12 | GSE292124 | GEO
Project description:Brown bear modern and palaeogenome study
