D39 wild-type vs D39 ΔplcR in GM17 medium
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ABSTRACT:
PROVIDER: PRJNA549688 | ENA |
REPOSITORIES: ENA
Similar Datasets
Project description:Comparison of the Streptococcus pneumoniae D39 wild-type vsD39 ΔplcR (SPD1745) in GM17medium
2019-07-31 | GSE133010 | GEO
Project description:Comparison of the Streptococcus pneumoniae D39 spxB- mutant vs D39. For DNA microarray analysis, D39 wild-type and two independently obtained D39spxB- mutants were grown as four, two and two biological replicates, respectively, in Glc-M17 under semi-aerobic conditions and harvested at an OD595 of approximately 0.25 (mid-exponential). The RNA of both D39spxB- strains was compared to D39 and the combined data was analyzed. All other procedures regarding microarray analyses were done as described before. A gene was considered differentially expressed when the fold change was ≥ 2, ≤ 0.5, with a Bayes p ≤ 0.00001 and when at least 7 measurements were available.
2013-08-08 | GSE45971 | GEO
Project description:Comparison of the Streptococcus pneumoniae D39 adcR mutant vs D39 wild type in CDM plus 0.2mM Zn One condition design comparison of two strains including a dye swap
2011-05-13 | E-GEOD-29234 | biostudies-arrayexpress
Project description:Comparison of the Streptococcus pneumoniae D39 spxB- mutant vs D39. For DNA microarray analysis, D39 wild-type and two independently obtained D39spxB- mutants were grown as four, two and two biological replicates, respectively, in Glc-M17 under semi-aerobic conditions and harvested at an OD595 of approximately 0.25 (mid-exponential). The RNA of both D39spxB- strains was compared to D39 and the combined data was analyzed. All other procedures regarding microarray analyses were done as described before. A gene was considered differentially expressed when the fold change was M-bM-^IM-% 2, M-bM-^IM-$ 0.5, with a Bayes p M-bM-^IM-$ 0.00001 and when at least 7 measurements were available. One condition design comparision of two strains including a dye swap.
2013-08-08 | E-GEOD-45971 | biostudies-arrayexpress
Project description:Comparison of the Streptococcus pneumoniae D39 cps mgtA mutant vs cps wild type
2011-05-05 | GSE26154 | GEO
Project description:By using the transcriptomic approach, we have elucidated the effect of Ni2+ on the global gene expression of S. pneumoniae D39 by identifying several differentially expressed genes/operons in the presence of a high extracellular concentration of Ni2+. The genes belonging to the AdcR regulon (adcRCBA, adcAII-phtD, phtA, phtB and phtE) and the PsaR regulon (pcpA, prtA and psaBCA) were highly upregulated in the presence of Ni2+. We have further studied the role of Ni2+ in the regulation of the AdcR regulon by using ICP-MS analysis, electrophoretic mobility shift assays and transcriptional lacZ-reporter studies, and demonstrate that Ni2+ is directly involved in the derepression of the AdcR regulon via the Zn2+-dependent repressor AdcR, and has an opposite effect on the expression of the AdcR regulon as compared to Zn2+. Comparison of the Streptococcus pneumoniae D39 wild-type vs D39 ÎadcR in CDM Plus 0.3 mM Ni2+ Two condition design comparison of Wild-type strain vs mutant strain including a dye swap
2015-11-20 | E-GEOD-73851 | biostudies-arrayexpress
Project description:Comparison of the Streptococcus pneumoniae D39 lacR mutant compared to D39 wild type in M17 medium+ 0.5 % (w/v) Glucose (GM17) Two condition design comparison of Wild-type strain including a dye swap
2014-06-20 | E-GEOD-58183 | biostudies-arrayexpress
Project description:Transcriptome comparison of the Streptococcus pneumoniae D39 wild-type grown in THY medium till OD620=0.25 and then treated with or without 1mM H2O2 for 40mins.
2017-07-27 | GSE65155 | GEO
Project description:Comparison of the Streptococcus pneumoniae D39 ulaR mutant compared to D39 wild type in M17 medium + 10mM ascorbic acid (AM17) One condition design comparision of two strains including a dye swap
2014-10-28 | E-GEOD-61649 | biostudies-arrayexpress
Project description:The objective of this comparison was to identify the impact of NADH on the transcriptome of Streptococcus pneumoniae D39. Transcriptome comparison of the D39 wild-type grown in chemically-defined medium (CDM) with 0 mg/ml NADH to 0.5 mg/ml NADH revealed elevated expression of various genes/operons (adhB1, fba, hemH, rex, gapN, nirC, pncB, gap, adhE, and adhB2) involved in the transport and biosynthesis of niacin. In this study, we investigated the transcriptomic response of Streptococcus pneumoniae D39 to NADH. Transcriptome comparison of the D39 wild-type grown in chemically-defined medium (CDM) with 0 mg/ml NADH to 0.5 mg/ml NADH revealed elevated expression of various genes/operons (adhB1, fba, hemH, rex, gapN, nirC, pncB, gap, adhE, and adhB2) involved in the transport and biosynthesis of niacin. Microarray results were further confirmed by β-galactosidase assays. Promoter-lacZ fusions assays and microarray studies showed that the transcriptional regulator, Rex acts as a transcriptional repressor of a number of genes/operons (adhB1, fba, hemH, rex, gapN, nirC, pncB, gap, adhE, and adhB2) involved in niacin uptake and biosynthesis in the presence of NADH. The putative operator site of Rex in the promoter regions of Rex-regulated genes is predicted and confirmed by promoter mutational experiments.
2018-06-01 | GSE94572 | GEO